Meta-Analysis of the Public RNA-Seq Data of the Western Honeybee to Construct Reference Transcriptome Data.

The Western honeybee () is valuable in biological research and agriculture. Its genome sequence was published before those for other insect species. RNA-Seq data for have been applied in several recently published studies. Nevertheless, these data have not been prepared for use in subsequent meta-analyses. To promote transcriptome analysis, we constructed reference transcriptome data using the reference genome sequence and RNA-Seq data curated from about 1,000 runs of public databases. The new reference transcriptome data construct comprised 149,685 transcripts, and 194,174 protein sequences were predicted. Approximately 50-60% of the predicted protein sequences were functionally annotated using the protein sequence data for several model and insect species. Novel candidate immune-related transcripts were searched by meta-analysis using immune-response-related RNA-Seq and reference transcriptome data. Three to twenty candidate transcripts including were upregulated or downregulated in response to both viral and bacterial infections. The constructed reference transcriptome data may facilitate future transcriptome analyses of .