Yokoi Kakeru, Hatakeyama Masatsugu, Kuwazaki Seigo, Maeda Taro, Yoshiyama Mikio, Horigane-Ogihara Mari, Matsuyama Shigeru, Jouraku Akiya, Bono Hidemasa, Kimura Kiyoshi
Insect Design Technology Group, Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, Japan.
Biodiversity Conservation and Utilization Group, Division of Agroecosystem Management Research, Institute of Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO), 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan.
Sci Data. 2025 Jun 2;12(1):926. doi: 10.1038/s41597-025-05279-z.
Comprehensive expression datasets were constructed for Apis mellifera and Apis cerana japonica. Firstly, we obtained RNA-Seq data of the samples prepared: post-oviposition day 6 to day 58 samples of A. mellifera workers (larva to adult), and day 9, 10, 12, and 13 samples of A. mellifera queen (larva to pupa), and day 9 to day 18 samples of A. cerana japonica workers (larva to adult). For A. cerana japonica, reference transcript sequence, predicted amino acid sequence, and functional annotation data were generated based on the genome sequence and RNA-Seq data. Using the transcript sequence and RNA-Seq data, comprehensive expression data for all transcripts of A. mellifera and A. cerana japonica were prepared. Hierarchical clustering analyses, the used sample preparation method, and other evaluation results ensured that both sets of expression data were reliable for use as comprehensive reference expression datasets. Therefore, these data can be used for honey bee research or comparative or evolutionary studies on insect species or social insect species at the genetic and molecular levels.
构建了西方蜜蜂和日本蜜蜂的综合表达数据集。首先，我们获得了所制备样本的RNA测序数据：西方蜜蜂工蜂（幼虫到成虫）产卵后第6天至第58天的样本，西方蜜蜂蜂王（幼虫到蛹）第9、10、12和13天的样本，以及日本蜜蜂工蜂（幼虫到成虫）第9天至第18天的样本。对于日本蜜蜂，基于基因组序列和RNA测序数据生成了参考转录本序列、预测的氨基酸序列和功能注释数据。利用转录本序列和RNA测序数据，制备了西方蜜蜂和日本蜜蜂所有转录本的综合表达数据。层次聚类分析、所使用的样本制备方法以及其他评估结果确保了这两组表达数据都可作为可靠的综合参考表达数据集使用。因此，这些数据可用于蜜蜂研究或在遗传和分子水平上对昆虫物种或社会性昆虫物种进行比较或进化研究。
