Key Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian 116081, China.
Int J Mol Sci. 2022 Oct 13;23(20):12229. doi: 10.3390/ijms232012229.
Seed germination is susceptible to external environmental factors, especially salt stress. is a halophyte with strong salt tolerance, and the germination rate of brown seeds under 1000 mM NaCl treatment still reached 28.9%. To explore the mechanism of salt stress response during brown seed germination in , we conducted transcriptomic analysis on the dry seeds (SlD), germinated seeds under the control condition (SlG_C), and salt treatment (SlG_N). Transcriptome analysis revealed that 13314 and 755 differentially expressed genes (DEGs) from SlD vs. SlG_C and SlG_C vs. SlG_N were detected, respectively. Most DEGs were enriched in pathways related to transcription regulation and hormone signal transduction, ROS metabolism, cell wall organization or biogenesis, and carbohydrate metabolic process in two contrasting groups. Compared with the control condition, POD and CAT activity, HO, soluble sugar, and proline contents were increased during germinated seeds under salt stress. Furthermore, functional analysis demonstrated that overexpression of significantly enhanced salt tolerance during the germination stage in . These results not only revealed the tolerant mechanism of brown seed germination in response to salinity stress but also promoted the exploration and application of salt-tolerant gene resources of .
种子萌发易受外界环境因素影响，特别是盐胁迫。是一种耐盐性很强的盐生植物，在 1000mM NaCl 处理下，棕色种子的发芽率仍达到 28.9%。为了探讨在盐胁迫下棕色种子萌发过程中的盐胁迫响应机制，我们对干种子（SlD）、对照条件下萌发的种子（SlG_C）和盐处理下萌发的种子（SlG_N）进行了转录组分析。转录组分析显示，SlD 与 SlG_C 相比和 SlG_C 与 SlG_N 相比分别检测到 13314 个和 755 个差异表达基因（DEGs）。在两个对比组中，大多数 DEGs 富集在与转录调控和激素信号转导、ROS 代谢、细胞壁组织或生物发生以及碳水化合物代谢过程相关的途径中。与对照条件相比，盐胁迫下萌发种子的 POD 和 CAT 活性、HO、可溶性糖和脯氨酸含量增加。此外，功能分析表明，在 中的过表达显著增强了种子萌发阶段的耐盐性。这些结果不仅揭示了棕色种子萌发对盐胁迫的耐受机制，也促进了盐生植物耐盐基因资源的探索和应用。
