基于 SAXS 的 DNA-SOD1 复合物形成的氧化还原依赖性研究
SAXS Examinations of the Redox-Dependent Formation of a DNA-SOD1 Complex.
机构信息
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, School of Chemistry, Central China Normal University, Wuhan 430079, China.
出版信息
Int J Mol Sci. 2022 Oct 21;23(20):12673. doi: 10.3390/ijms232012673.
Cu/Zn superoxide dismutase (SOD1) plays a key role in the maintenance of cellular reactive oxygen species (ROS) homeostasis as an antioxidant enzyme. We recently found that SOD1 is involved in the regulation of gene expression in response to changes in cellular ROS levels by binding to DNA-specific sequences. Moreover, the SOD1 binding to DNA was observed to be redox-dependent in solutions. Thus, we examined the redox-dependent DNA binding of SOD1 by multiple measurements, including small-angle X-ray scattering (SAXS), indicating the redox-dependent formation of a DNA-SOD1 complex in solutions. The redox-dependent formation of the DNA-SOD1 complex could underlie the SOD1 regulation of gene expression.
铜/锌超氧化物歧化酶（SOD1）作为一种抗氧化酶，在维持细胞活性氧（ROS）稳态方面发挥着关键作用。我们最近发现，SOD1 通过与 DNA 特异性序列结合，参与细胞内 ROS 水平变化时的基因表达调控。此外，在溶液中观察到 SOD1 与 DNA 的结合具有氧化还原依赖性。因此，我们通过多种测量方法，包括小角 X 射线散射（SAXS），研究了 SOD1 的氧化还原依赖性 DNA 结合，表明在溶液中形成了氧化还原依赖性的 DNA-SOD1 复合物。DNA-SOD1 复合物的氧化还原依赖性形成可能是 SOD1 调节基因表达的基础。
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