Xiao Chenwen, Li Lin, Liu Yan, Huang Yee, Wang Yanli, Wang Jiaoyu, Bao Guolian, Sun Guochang, Lin Fucheng
State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
J Fungi (Basel). 2022 Sep 25;8(10):1006. doi: 10.3390/jof8101006.
is an important zoonotic dermatophyte, which seriously harms the skin of humans and animals. Chemical drugs are generally used for the prevention and treatment of the disease caused by . . Discovering new compounds from natural products is an important approach for new drug development. includes a variety of fungal species used for biological control of phytopathogenic fungi. However, the antifungal effects of and their metabolites on zoonotic fungal pathogens are largely unknown. Here, the effect of trichodermin, a metabolite derived from the plant endophytic fungus , on was examined, and the underlying mechanism was explored. growth was suppressed significantly by trichodermin and completely inhibited under 1000 μg/mL trichodermin. The production and germination of spores were remarkably reduced upon exposure to trichodermin, in comparison with control samples. Treatment of lesions caused by on the rabbit skin with 1 mg/mL trichodermin prompted the healing process significantly; however, 20 mg/mL trichodermin was likely toxic to the skin. Under trichodermin treatment, the number of mitochondria in increased significantly, while a few mitochondria-related genes decreased, indicating possible mitochondrial damage. In transcriptome analysis, the GO terms enriched by DEGs in the trichodermin-treated group included carbohydrate metabolic process, integral component of membrane, intrinsic component of membrane, and carbohydrate binding, while the enriched KEGG pathways comprised biosynthesis of secondary metabolites, glycolysis/gluconeogenesis, and carbon metabolism. By comparing the wild type and a gene deletion strain of , we found that , an ABC transporter encoding gene, was involved in sensitivity to trichodermin.
是一种重要的人畜共患皮肤癣菌，严重危害人和动物的皮肤。化学药物通常用于预防和治疗由……引起的疾病。从天然产物中发现新化合物是新药开发的重要途径。……包括多种用于植物病原真菌生物防治的真菌物种。然而，……及其代谢产物对人畜共患真菌病原体的抗真菌作用在很大程度上尚不清楚。在此，研究了植物内生真菌……产生的代谢产物曲古抑菌素对……的作用，并探讨了其潜在机制。曲古抑菌素显著抑制……生长，在1000μg/mL曲古抑菌素作用下完全抑制。与对照样品相比，暴露于曲古抑菌素后，……孢子的产生和萌发显著减少。用1mg/mL曲古抑菌素处理兔皮肤上由……引起的病变，显著促进了愈合过程；然而，20mg/mL曲古抑菌素可能对皮肤有毒。在曲古抑菌素处理下，……中线粒体数量显著增加，而一些与线粒体相关的基因减少，表明可能存在线粒体损伤。在转录组分析中，曲古抑菌素处理组中差异表达基因富集的GO术语包括碳水化合物代谢过程、膜的整合成分、膜的内在成分和碳水化合物结合，而富集的KEGG途径包括次生代谢物的生物合成、糖酵解/糖异生和碳代谢。通过比较……的野生型和基因缺失菌株，我们发现……（一个编码ABC转运蛋白的基因）参与了……对曲古抑菌素的敏感性。
