Shentu Xuping, Zhan Xiaohuan, Ma Zheng, Yu Xiaoping, Zhang Chuanxi
Institute of Insect Science Zhejiang University Hangzhou China ; College of Life Science China Jiliang University Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine Hangzhou China.
College of Life Science China Jiliang University Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine Hangzhou China.
Braz J Microbiol. 2014 May 2;45(1):248-54. doi: 10.1590/S1517-83822014005000036. eCollection 2014.
The endophytic fungus strain 0248, isolated from garlic, was identified as Trichoderma brevicompactum based on morphological characteristics and the nucleotide sequences of ITS1-5.8S- ITS2 and tef1. The bioactive compound T2 was isolated from the culture extracts of this fungus by bioactivity-guided fractionation and identified as 4β-acetoxy-12,13- epoxy-Δ(9)-trichothecene (trichodermin) by spectral analysis and mass spectrometry. Trichodermin has a marked inhibitory activity on Rhizoctonia solani, with an EC50 of 0.25 μg mL(-1). Strong inhibition by trichodermin was also found for Botrytis cinerea, with an EC50 of 2.02 μg mL(-1). However, a relatively poor inhibitory effect was observed for trichodermin against Colletotrichum lindemuthianum (EC50 = 25.60 μg mL(-1)). Compared with the positive control Carbendazim, trichodermin showed a strong antifungal activity on the above phytopathogens. There is little known about endophytes from garlic. This paper studied in detail the identification of endophytic T. brevicompactum from garlic and the characterization of its active metabolite trichodermin.
从大蒜中分离出的内生真菌菌株0248，根据其形态特征以及ITS1-5.8S-ITS2和tef1的核苷酸序列，被鉴定为短密木霉。通过生物活性导向分级分离从该真菌的培养提取物中分离出生物活性化合物T2，并通过光谱分析和质谱鉴定为4β-乙酰氧基-12,13-环氧-Δ(9)-单端孢霉烯（单端孢菌素）。单端孢菌素对茄丝核菌具有显著的抑制活性，其EC50为0.25 μg mL(-1)。还发现单端孢菌素对灰葡萄孢有较强的抑制作用，其EC50为2.02 μg mL(-1)。然而，观察到单端孢菌素对菜豆炭疽菌的抑制作用相对较弱（EC50 = 25.60 μg mL(-1)）。与阳性对照多菌灵相比，单端孢菌素对上述植物病原菌表现出较强的抗真菌活性。关于大蒜内生菌的了解很少。本文详细研究了大蒜内生短密木霉的鉴定及其活性代谢产物单端孢菌素的特性。
