Ali Saqib, Farooq Imran, Bugshan Amr, Siddiqui Intisar Ahmad, Al-Khalifa Khalifa S, Al-Hariri Mohammed
Department of Biomedical Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, KSA.
Department of Quality Assurance, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, KSA.
J Taibah Univ Med Sci. 2020 Dec 14;16(2):283-287. doi: 10.1016/j.jtumed.2020.10.022. eCollection 2021 Apr.
In this study, we aimed to analyse the enamel-remineralisation potential of propolis.
Twenty enamel blocks (N = 20) were randomly divided into two groups (n = 10). In group 1 (control), enamel blocks were brushed with artificial saliva (AS). In group 2, they were brushed with propolis oil. All the blocks were demineralised by exposing them to 6 wt% citric acid (pH: 2.2) for 5 min. Brushing was performed inside a tooth brushing simulation machine with manual toothbrushes. Each sample received 5,000 linear strokes. Surface microhardness analysis was performed for each sample at three time intervals (pre-demineralisation or baseline, post-demineralisation, and post-remineralisation) to obtain the Vickers hardness numbers (VHNs).
An enhancement in the microhardness of the enamel samples was observed after brushing with propolis oil when compared with brushing using AS alone. In group 1 (control group), the mean baseline VHN was 583.66. It decreased to 116.23 after demineralisation and increased to 184.02 after remineralisation. The mean baseline VHN of group 2 was 506.91. It decreased to 317.60 after demineralisation and increased to 435.19 after remineralisation. The VHN values of both the groups revealed statistically significant differences ( < 0.05) in inter-group and intra-group comparisons.
Brushing of enamel blocks with propolis led to a greater enhancement in their microhardness levels when compared with the control group. Future studies are essential to validate the exact mechanism of the beneficial effects of propolis on enamel.
在本研究中，我们旨在分析蜂胶对牙釉质再矿化的潜力。
将20个牙釉质块（N = 20）随机分为两组（n = 10）。在第1组（对照组）中，用人工唾液（AS）刷洗牙釉质块。在第2组中，用蜂胶油刷洗。将所有牙釉质块暴露于6 wt%柠檬酸（pH：2.2）中5分钟进行脱矿。在刷牙模拟机内用手动牙刷进行刷牙。每个样品接受5000次线性刷动。在三个时间间隔（脱矿前或基线、脱矿后和再矿化后）对每个样品进行表面显微硬度分析，以获得维氏硬度值（VHNs）。
与仅使用人工唾液刷牙相比，用蜂胶油刷牙后观察到牙釉质样品的显微硬度有所提高。在第1组（对照组）中，平均基线维氏硬度值为583.66。脱矿后降至116.23，再矿化后升至184.02。第2组的平均基线维氏硬度值为506.91。脱矿后降至317.60，再矿化后升至435.19。两组的维氏硬度值在组间和组内比较中均显示出统计学显著差异（P < 0.05）。
与对照组相比，用蜂胶刷洗牙釉质块导致其显微硬度水平有更大提高。未来的研究对于验证蜂胶对牙釉质有益作用的确切机制至关重要。
