Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to Infection and Transfection.

The role of α-enolase (ENO1) in -related gastric lesions might be a critical factor in the pathogenesis, but remains undefined. This study investigated the differential expression of α-enolase in clinical gastric specimens and cultured normal/cancer cells in response to () infection and transfection using qPCR, Western blots and histochemical methods. A total of 172 gastric specimens were collected from 142 patients, the former comprising chronic superficial gastritis (CSG), precancerous diseases (PCDs, including atrophic gastritis, intestinal metaplasia and dysplasia) and gastric cancer (GC) cases. Among the CSG and PCD cases, the -infected group had significantly elevated ENO1 mRNA levels compared with the uninfected group. In the GC cases, differential ENO1 expressions were detected between the cancer tissues and the paracancerous tissues. Notably, significant difference was first detected between the GC cell (AGS) and the normal cell (GES-1) as a response of ENO1 to infection and transfection. This report reveals that ENO1 expression is associated with infection, transfection, co-culture duration, multiplicity of infection, gastric normal/cancerous cell lines and cellular differentiation. The findings may be crucial bases for further ascertaining pathogenic mechanism and formulating novel therapeutic and diagnostic strategies.