Mantha Olivier L, Hankard Régis, Tea Illa, Schiphorst Anne-Marie, Dumas Jean-François, Berger Virginie, Goupille Caroline, Bougnoux Philippe, De Luca Arnaud
Nutrition, Growth and Cancer (N2C) UMR 1069, University of Tours, INSERM, 37032 Tours, France.
Nantes University, CNRS, CEISAM, UMR6230, F-44000 Nantes, France.
Metabolites. 2022 Sep 24;12(10):899. doi: 10.3390/metabo12100899.
While clinical evidence remains limited, an extensive amount of research suggests a beneficial role of n-3 polyunsaturated fatty acid supplementation in cancer treatment. One potential benefit is an improvement of protein homeostasis, but how protein metabolism depends on proinflammatory cytokines in this context remains unclear. Here, using the natural abundance of the stable isotopes of nitrogen as a marker of changes in protein metabolism during a randomized, double-blind, controlled clinical trial, we show that protein homeostasis is affected way faster than proinflammatory cytokines in metastatic breast cancer patients supplemented with n-3 polyunsaturated fatty acids. We provide some evidence that this response is unrelated to major changes in whole-body substrate oxidation. In addition, we demonstrate that more fatty acids were impacted by metabolic regulations than by differences in their intake levels during the supplementation. This study documents that the percentage of patients that complied with the supplementation decreased with time, making compliance assessment crucial for the kinetic analysis of the metabolic and inflammatory responses. Our results highlight the time-dependent nature of metabolic and inflammatory changes during long-chain n-3 fatty acid supplementation.
虽然临床证据仍然有限，但大量研究表明补充n-3多不饱和脂肪酸在癌症治疗中具有有益作用。一个潜在的益处是改善蛋白质稳态，但在这种情况下蛋白质代谢如何依赖促炎细胞因子仍不清楚。在这里，我们在一项随机、双盲、对照临床试验中，利用氮稳定同位素的自然丰度作为蛋白质代谢变化的标志物，表明在补充n-3多不饱和脂肪酸的转移性乳腺癌患者中，蛋白质稳态受到影响的速度比促炎细胞因子快得多。我们提供了一些证据表明这种反应与全身底物氧化的主要变化无关。此外，我们证明在补充期间，受代谢调节影响的脂肪酸比受其摄入水平差异影响的脂肪酸更多。这项研究记录了随着时间推移，遵守补充方案的患者百分比下降，这使得依从性评估对于代谢和炎症反应的动力学分析至关重要。我们的结果突出了长链n-3脂肪酸补充期间代谢和炎症变化的时间依赖性。
