Chen Hsin-Yu, Chen Yung-Tsung, Li Kuan-Yi, Huang Hsiao-Wen, Lin Yu-Chun, Chen Ming-Ju
Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
Department of Food Science, National Taiwan Ocean University, Keelung 202301, Taiwan.
Microorganisms. 2022 Sep 21;10(10):1881. doi: 10.3390/microorganisms10101881.
Atopic dermatitis (AD) is a chronic and relapsing inflammatory skin disease accompanied with severe itching and skin lesions. Current studies have demonstrated that probiotics can exert an immunomodulatory effect, improve epithelial barrier function, and normalize the composition of gut microbiota. Thus, the aim of this study was to investigate the effect of probiotics on the immune balance of AD in vivo. We first screened two lactic acid bacteria strains, which were subsp. MP01 and subsp. MP02, from 10 strains isolated from traditional fermented milk with inflammation regulating activities in vitro. In the house dust mite (HDM) extraction-induced AD mouse model, mice were assigned randomly to four groups: control group (PC), HDM-induced AD group (NC), HDM-induced AD mice with administration of a mixture of heat-killed MP01 and MP02 at a low concentration (LD), and high concentration (HD) groups. Compared with the NC group, the probiotic treatments could relieve the AD symptoms. Moreover, the LD group significantly decreased total and HDM-specific IgE concentration. These results indicated that a combination of heat-killed MP01 and MP02 strains modulated the proportion of IL4CD4 T cells and IFNγCD4 T cells in the spleen of HDM extraction-induced AD mice. In conclusion, administration of the heat-killed MP01 and MP02 mixtures appeared to relieve the classic AD signs, decrease serum IgE concentration, and rebalance the population of Th1/Th2 cells in HDM extraction-induced AD mice. The immunomodulatory activities of a combination of heat-killed MP01 and MP02 provided a potential new therapeutic strategy against AD.
特应性皮炎（AD）是一种慢性复发性炎症性皮肤病，伴有严重瘙痒和皮肤损伤。目前的研究表明，益生菌可以发挥免疫调节作用，改善上皮屏障功能，并使肠道微生物群的组成正常化。因此，本研究的目的是在体内研究益生菌对AD免疫平衡的影响。我们首先从10株从具有体外炎症调节活性的传统发酵乳中分离出的菌株中筛选出两株乳酸菌菌株，即MP01亚种和MP02亚种。在屋尘螨（HDM）提取物诱导的AD小鼠模型中，将小鼠随机分为四组：对照组（PC）、HDM诱导的AD组（NC）、低浓度（LD）和高浓度（HD）组，其中LD组和HD组为HDM诱导的AD小鼠并给予热灭活的MP01和MP02混合物。与NC组相比，益生菌治疗可缓解AD症状。此外，LD组显著降低了总IgE和HDM特异性IgE浓度。这些结果表明，热灭活的MP01和MP02菌株组合调节了HDM提取物诱导的AD小鼠脾脏中IL4⁺CD4⁺ T细胞和IFNγ⁺CD4⁺ T细胞的比例。总之，给予热灭活的MP01和MP02混合物似乎可以缓解经典的AD症状，降低血清IgE浓度，并使HDM提取物诱导的AD小鼠中Th1/Th2细胞群重新平衡。热灭活的MP01和MP02组合的免疫调节活性为AD提供了一种潜在的新治疗策略。
