Zhang Yao, Jiang Zhilin, Li Yunran, Feng Zhiping, Zhang Xian, Zhou Ruiping, Liu Chao, Yang Lijuan
College of Bioengineering, Sichuan University of Science & Engineering, Yibin 644000, China.
Liquor Making Bio-Technology & Application of Key Laboratory of Sichuan Province, Sichuan University of Science & Engineering, Yibin 644000, China.
Microorganisms. 2022 Sep 22;10(10):1889. doi: 10.3390/microorganisms10101889.
Feruloyl esterase (FAE)-producing micro-organisms to obtain ferulic acid (FA) from natural substrates have good industrial prospects, and the synergistic effect of multiple bacteria can better improve the yield of FA. In this study, on the premise of the synergistic effect of FAE, hemicellulose, and cellulase, the key strain with FAE was combined with CMCase and Xylanase-producing strains to produce FA. The combination of strains with higher FA production are , , , and their FA production can reach 109.67 μg/g, which is 15% higher than that of single bacteria. To explore the effects of temperature, Ph, inoculum amount, distillers grains concentration and fermentation time on the FAE activity of the combination of strains in the fermentation process, and determined that temperature, Ph, and fermentation time were the main influencing factors and optimized through orthogonal design. The optimized fermentation conditions are 34 °C, Ph 8.0, and fermentation days for 6 days, the FAE activity can reach 270.78 U/L, and the FA yield of the combined strain is 324.50 μg/g, which is 200% higher than that of single-strain fermentation.
利用产阿魏酸酯酶（FAE）的微生物从天然底物中获取阿魏酸（FA）具有良好的工业前景，多种细菌的协同作用能更好地提高FA的产量。本研究在FAE、半纤维素和纤维素酶协同作用的前提下，将产FAE的关键菌株与产羧甲基纤维素酶（CMCase）和木聚糖酶的菌株组合来生产FA。产FA较高的菌株组合为 、 、 ，其FA产量可达109.67μg/g，比单一细菌高出15%。为探究温度、pH、接种量、酒糟浓度和发酵时间对发酵过程中菌株组合FAE活性的影响，并通过正交设计确定温度、pH和发酵时间为主要影响因素并进行优化。优化后的发酵条件为34℃、pH 8.0、发酵6天，FAE活性可达270.78U/L，组合菌株的FA产量为324.50μg/g，比单菌株发酵高出200%。
