Zhang Yao, Feng Zhiping, Xiang Hongzhu, Zhang Xian, Yang Lijuan
College of Bioengineering, Sichuan University of Science & Engineering, Yibin 644000, China.
Liquor Making Bio-Technology & Application of Key Laboratory of Sichuan Province, Sichuan University of Science & Engineering, Yibin 644000, China.
Microorganisms. 2023 Apr 10;11(4):989. doi: 10.3390/microorganisms11040989.
Feruloyl esterase (EC3.1.1.73; FAE) can degrade biomass to release ferulic acid (FA), which has a high application in bioprocessing, food, pharmaceutical, paper, feed, and other industrial fields. A strain of Z28 with ferulic esterase activity was screened from Daqu. In addition, the FAE gene was expressed in BL21 (DE3). The enzyme consists of 340 amino acids with a molecular mass of 37.7 kDa. The FAE enzyme activity was 463 U/L when the substrate was ethyl 4-hydroxy-3-methoxycinnamate and the optimum temperature and pH were 50 °C and 8.0, respectively. The enzyme had good stability at temperatures of 25-40 °C and a pH of 8.0. Ba, Cu, Mn, and Ca had a strong inhibitory effect on the enzyme activity, and Na had a promotive effect on the enzyme activity. The de-starching wheat bran was degraded by KoFAE, and the FA release was up to 227.15 µg/g. This indicated that the heterologous expression of KoFAE from Z28 in had a certain potential of biodegradation, which can be applied to the degradation of agricultural waste to obtain high value-added FA products.
阿魏酸酯酶（EC3.1.1.73；FAE）能够降解生物质以释放阿魏酸（FA），阿魏酸在生物加工、食品、制药、造纸、饲料及其他工业领域具有很高的应用价值。从大曲中筛选出一株具有阿魏酸酯酶活性的菌株Z28。此外，FAE基因在BL21（DE3）中得到表达。该酶由340个氨基酸组成，分子量为37.7 kDa。以4-羟基-3-甲氧基肉桂酸乙酯为底物时，FAE酶活性为463 U/L，最适温度和pH分别为50℃和8.0。该酶在25 - 40℃、pH为8.0时具有良好的稳定性。Ba、Cu、Mn和Ca对酶活性有强烈的抑制作用，而Na对酶活性有促进作用。KoFAE可降解脱淀粉麦麸，阿魏酸释放量高达227.15 μg/g。这表明Z28的KoFAE在异源表达中具有一定的生物降解潜力，可应用于农业废弃物的降解以获得高附加值的阿魏酸产品。
