Wanderley Porto Maria Carolina, de Souza de Azevedo Pamela Oliveira, Lourenço Felipe Rebello, Converti Attilio, Vitolo Michele, Oliveira Ricardo Pinheiro de Souza
Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580, São Paulo 05508900, Brazil.
Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580, São Paulo 05508900, Brazil.
Microorganisms. 2022 Sep 24;10(10):1898. doi: 10.3390/microorganisms10101898.
was cultivated in MRS medium supplemented or not with polydextrose under different conditions in order to evaluate its effect on cell growth, lactic acid and bacteriocin-like inhibitory substance (BLIS) production. Independent variables were pH (4.0, 5.0, 6.0), rotational speed (50, 100, 150 rpm), polydextrose concentration (0.5, 1.0, 1.5%) and temperature (25, 30, 35 °C), while cell concentration and productivity after 24 h, maximum specific growth rate, specific rate of substrate (glucose) consumption, volumetric and specific lactic acid productivities, yields of biomass and lactic acid on consumed substrate were the dependent. The maximum cell concentration (10.24 ± 0.16 g L) and productivity (0.42 ± 0.01 g L h) were achieved at pH 6.0, 35 °C, 150 rpm using 1.5% polydextrose, while the maximum specific growth rate (0.99 ± 0.01 h) and yield of biomass (2.96 ± 0.34 g g) were achieved at the same pH and polydextrose concentration, but at 25 °C and 50 rpm. The specific substrate consumption rate (0.09 ± 0.02 g g h) and the volumetric lactic acid productivity (0.44 ± 0.02 g L h) were maximized at pH 6.0, 35 °C, 50 rpm and 0.5% polydextrose. BLIS produced in this last run displayed the highest antibacterial activity against , while the same activity was displayed against using 1.5% polydextrose. These results appear to be quite promising in view of possible production of this BLIS as an antibacterial agent in the food industry.
在不同条件下，将其接种于添加或不添加聚葡萄糖的MRS培养基中培养，以评估其对细胞生长、乳酸和类细菌素抑制物质（BLIS）产生的影响。自变量为pH值（4.0、5.0、6.0）、转速（50、100、150转/分钟）、聚葡萄糖浓度（0.5%、1.0%、1.5%）和温度（25、30、35℃），而24小时后的细胞浓度和生产率、最大比生长速率、底物（葡萄糖）消耗比速率、体积乳酸生产率和特定乳酸生产率、消耗底物上的生物量和乳酸产量为因变量。在pH值6.0、35℃、150转/分钟、使用1.5%聚葡萄糖的条件下，达到了最大细胞浓度（10.24±0.16克/升）和生产率（0.42±0.01克/升·小时），而在相同pH值和聚葡萄糖浓度下，但在25℃和50转/分钟时，达到了最大比生长速率（0.99±0.01小时）和生物量产量（2.96±0.34克/克）。在pH值6.0、35℃、50转/分钟和0.5%聚葡萄糖的条件下，底物消耗比速率（0.09±0.02克/克·小时）和体积乳酸生产率（0.44±0.02克/升·小时）达到最大值。最后一次实验中产生的BLIS对……显示出最高的抗菌活性，而使用1.5%聚葡萄糖时对……显示出相同的活性。鉴于在食品工业中可能将这种BLIS作为抗菌剂生产，这些结果似乎很有前景。
