Ezzedine Jade A, Scheifler Mathilde, Desdevises Yves, Jacquet Stéphan
Université Savoie Mont-Blanc, INRAE, CARRTEL, 74200 Thonon les Bains, France.
Laboratoire de Physiologie Cellulaire et Végétale, CNRS, CEA, INRAE, IRIG, Université Grenoble Alpes, 38058 Grenoble, France.
Microorganisms. 2022 Sep 30;10(10):1960. doi: 10.3390/microorganisms10101960.
and like organisms (BALOs) are obligate bacterial predators of other Gram-negative bacteria. Here, we used quantitative PCR (qPCR) and recently developed specific primers which target the 16S rRNA gene to explore the abundance and distribution of three families of BALO belonging to the Oligoflexia class (i.e., , and over one year in the epilimnion and hypolimnion of Lakes Annecy and Geneva. was the dominant group at all sampling points except at the bottom of Lake Geneva, where was found in higher number. In addition, the abundance of BALOs increased significantly during the warmer months. Using high-throughput sequencing (Illumina Miseq), hundreds of OTUs were identified for and . Phylogenetic analysis suggests that are more diverse than and that some OTUs belong to new species of . We also found that dominant OTUs were present simultaneously in the two lakes, while some others were specific to each lake, suggesting an adaptive pattern. Finally, both abundance and diversity of BALOs were poorly associated with abiotic factors except temperature, suggesting the importance of studying biotic relationships, assumed to play a greater role than physico-chemical variables in BALOs' dynamics and distribution.
噬菌蛭弧菌属生物（BALOs）是其他革兰氏阴性菌的专性细菌捕食者。在此，我们使用定量聚合酶链反应（qPCR）和最近开发的靶向16S rRNA基因的特异性引物，对阿讷西湖和日内瓦湖的湖上层和湖下层中属于噬纤维菌纲的三个噬菌蛭弧菌属生物家族（即噬纤维菌属、蛭弧菌属和嗜盐菌属）的丰度和分布进行了为期一年的研究。除了在日内瓦湖湖底发现噬盐菌属数量较多外，噬纤维菌属在所有采样点都是优势菌群。此外，噬菌蛭弧菌属生物的丰度在温暖月份显著增加。使用高通量测序（Illumina Miseq），为噬纤维菌属和嗜盐菌属鉴定出了数百个操作分类单元（OTUs）。系统发育分析表明，噬纤维菌属比嗜盐菌属更多样化，并且一些OTUs属于噬纤维菌属的新物种。我们还发现，优势OTUs在两个湖泊中同时存在，而其他一些则是每个湖泊特有的，这表明存在一种适应性模式。最后，除了温度外，噬菌蛭弧菌属生物的丰度和多样性与非生物因素的相关性较差，这表明研究生物关系很重要，据推测生物关系在噬菌蛭弧菌属生物的动态和分布中比物理化学变量发挥更大的作用。
