College of Food Science and Engineering, Jilin University, Changchun 130062, China.
Molecules. 2022 Oct 21;27(20):7136. doi: 10.3390/molecules27207136.
Antimicrobial Photodynamic Treatment (aPDT) is a non-thermal sterilization technology, which can inactivate common foodborne pathogens. In the present study, photodynamic inactivation on () with different concentrations of curcumin and light dose was evaluated and the mechanisms were also investigated. The results showed that curcumin-based aPDT could inactivate cells by 6.9 log CFU/mL in phosphate buffered saline (PBS). Moreover, the modified Gompertz model presented a good fit at the inactivation data of . Photodynamic treatment caused cell membrane damage as revealed by analyzing scanning electron microscopy (SEM) images. Leakage of intracellular constituents further indicated that cell membrane permeability was changed. Flow cytometry with double staining demonstrated that cell membrane integrity and the activity of nonspecific esterase were destroyed. Compared with the control group, intracellular reactive oxygen species (ROS) levels caused by photodynamic treatment significantly increased. Furthermore, curcumin-based aPDT reduced by 5 log CFU/mL in juices. The color of the juices was also tested using a Chromatic meter, and it was found that * values were the most markedly influenced by photodynamic treatment. Overall, curcumin-based aPDT had strong antibacterial activity against . This approach has the potential to remove foodborne pathogens from liquid food.
抗菌光动力疗法（aPDT）是一种非热灭菌技术，可以灭活常见的食源性病原体。本研究评价了不同浓度姜黄素和光照剂量对（）的光动力灭活作用，并探讨了其作用机制。结果表明，基于姜黄素的 aPDT 可以在磷酸盐缓冲盐水（PBS）中使细胞失活 6.9 log CFU/mL。此外，修正的 Gompertz 模型很好地拟合了的灭活数据。光动力处理导致细胞膜损伤，通过分析扫描电子显微镜（SEM）图像可以看出。细胞内成分的泄漏进一步表明细胞膜通透性发生了变化。用双重染色的流式细胞术表明，细胞膜完整性和非特异性酯酶的活性被破坏。与对照组相比，光动力处理引起的细胞内活性氧（ROS）水平显著增加。此外，基于姜黄素的 aPDT 在果汁中使减少了 5 log CFU/mL。还使用色度计测试了果汁的颜色，发现光动力处理对*值的影响最为显著。总体而言，基于姜黄素的 aPDT 对具有很强的抗菌活性。这种方法有可能从液体食品中去除食源性病原体。
