Fan L Y, Sun C L, Chen Y H, Gao G S
Huamei Hospital, University of Chinese Academy of Sciences, Zhejiang Provincial Key Laboratory of Diagnosis, Treatment and Research of Digestive System Tumors, Ningbo 315010, China.
Zhejiang University-University of Edinburgh Institute, Haining 314400, China.
Zhonghua Gan Zang Bing Za Zhi. 2022 Sep 20;30(9):954-961. doi: 10.3760/cma.j.cn501113-20211014-00509.
To analyze guanine nucleotide-binding protein subunit beta-2-like 1 (GNB2L1) expression based on bioinformatics, so as to evaluate its role and its relationship with survival rate during the occurrence and development of hepatocellular carcinoma. GEPIA, UALCAN and HPA databases were used to analyze the expression level of GNB2L1 and its relationship with HCC survival rate. Mutations in the GNB2L1 gene and their impact on survival were analyzed using the cBioPortal database. LinkedOmics database was used to analyze GNB2L1-related genes in HCC. Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were performed simultaneously. STEING database was used to construct the GNB2L1 protein interaction network. TIMER database was used to analyze the relationship between GNB2L1 gene expression and immune infiltration in hepatocellular carcinoma. Differential expression of GNB2L1 in plasma platelets of HCC patients and healthy controls was analyzed using mRNA-based sequencing technology. Data between groups were compared using an independent-samples -test. GNB2L1 expression level was significantly increased in HCC tissues (<0.05), and its expression was significantly correlated with body weight, classification and stage (<0.05). The overall survival rate was higher in GNB2L1 low expression group (<0.001). GNB2L1 and its related genes were related to biological process regulation, metabolic process, protein binding, oxidative phosphorylation, JAK-STAT signaling pathway, Ras signaling pathway and so on. GNB2L1 had interaction with RPS12, RPS11 and RPL19, and participated in multiple biological processes such as liver regeneration and positive regulation of endogenous apoptotic signaling pathway. GNB2L1 expression was significantly positively correlated with the infiltration degree of various immune cells in HCC (<0.05). Cox regression analysis showed that GNB2L1 was an independent risk factor for lower survival rate in patients with HCC [Hazard ratio (95% confidence interval)=1.456 (1.034~2.051), =0.031]. GNB2L1expression levels were significantly higher in platelets of HCC patients than that of healthy controls (10.40±1.36 . 9.58±0.51, =2.194, =0.037). GNB2L1 has high expression and close relationship to survival rate in HCC. Therefore, GNB2L1 may be a potential biomarker of HCC.
基于生物信息学分析鸟嘌呤核苷酸结合蛋白β-2样1（GNB2L1）的表达，以评估其在肝细胞癌发生发展过程中的作用及其与生存率的关系。使用GEPIA、UALCAN和HPA数据库分析GNB2L1的表达水平及其与肝癌生存率的关系。使用cBioPortal数据库分析GNB2L1基因的突变及其对生存的影响。使用LinkedOmics数据库分析肝癌中与GNB2L1相关的基因。同时进行基因本体（GO）功能注释和京都基因与基因组百科全书通路富集分析。使用STEING数据库构建GNB2L1蛋白相互作用网络。使用TIMER数据库分析GNB2L1基因表达与肝细胞癌免疫浸润之间的关系。使用基于mRNA的测序技术分析肝癌患者和健康对照者血浆血小板中GNB2L1的差异表达。组间数据比较采用独立样本t检验。GNB2L1在肝癌组织中的表达水平显著升高（<0.05），其表达与体重、分级和分期显著相关（<0.05）。GNB2L1低表达组的总生存率较高（<0.001）。GNB2L1及其相关基因与生物过程调控、代谢过程、蛋白质结合、氧化磷酸化、JAK-STAT信号通路、Ras信号通路等有关。GNB2L1与RPS12、RPS11和RPL19相互作用，并参与肝再生和内源性凋亡信号通路的正调控等多个生物学过程。GNB2L1表达与肝癌中各种免疫细胞的浸润程度显著正相关（<0.05）。Cox回归分析表明，GNB2L1是肝癌患者生存率降低的独立危险因素[风险比（95%置信区间）=1.456（1.034~2.051），P=0.031]。肝癌患者血小板中GNB2L1表达水平显著高于健康对照者（10.40±1.36 vs 9.58±0.51，t=2.194，P=0.037）。GNB2L1在肝癌中表达高且与生存率密切相关。因此，GNB2L1可能是肝癌的潜在生物标志物。
