Smart Nanobiodevices Group, Institute for Bioengineering of Catalonia, Barcelona, Spain.
Centro de Biotecnológia Animal SERIDA-DEVA-GIJON, Gijón, Spain.
Front Endocrinol (Lausanne). 2022 Oct 10;13:957684. doi: 10.3389/fendo.2022.957684. eCollection 2022.
Cooperative behaviour of sperm is one of the mechanisms that plays a role in sperm competition. It has been observed in several species that spermatozoa interact with each other to form agglomerates or bundles. In this study, we investigate the effect of physical and biochemical factors that will most likely promote bundle formation in bull sperm. These factors include fluid viscosity, swim-up process, post-thaw incubation time and media additives which promote capacitation. While viscosity does not seem to influence the degree of sperm bundling, swim-up, post-thaw migration time and suppressed capacitation increase the occurrence of sperm bundles. This leads to the conclusion that sperm bundling is a result of hydrodynamic and adhesive interactions between the cells which occurs frequently during prolonged incubation times.
精子的合作行为是精子竞争中起作用的机制之一。已经在几种物种中观察到，精子之间相互作用形成聚集体或束。在这项研究中，我们研究了最有可能促进公牛精子形成束的物理和生化因素的影响。这些因素包括促进获能的流体粘度、泳动过程、解冻后孵育时间和添加物。虽然粘度似乎不会影响精子束的程度，但泳动、解冻后迁移时间和抑制获能增加了精子束的发生。这得出结论，精子束是细胞之间的流体动力学和粘附相互作用的结果，这种相互作用在长时间孵育过程中经常发生。
