Peng Shudong, Guo Chaoqun, Wu Songjie, Duan Zhi
School of Food Science and Engineering, South China University of Technology, Guangzhou, China.
Guangdong Youmei Institute of Intelligent Bio-Manufacturing, Foshan, 528225, China.
Heliyon. 2022 Oct 17;8(10):e11125. doi: 10.1016/j.heliyon.2022.e11125. eCollection 2022 Oct.
The purpose of this study was to isolate exopolysaccharides (EPS) from lactic acid bacteria (LAB) and evaluate EPS anti-UVB viability. VHPriobi O17 with high EPS production was screened from 34 strains of LAB. The EPS (OP-2) produced by VHPriobi O17 was purified by alcohol precipitation and DEAE-μSphere anion exchange chromatography. By ion chromatography, FT-IR spectrum and gel column chromatography, EPS (OP-2) was a novel Man-like polysaccharide with the weight-averaged molecular of 84.2 kDa. The EPS (OP-2) can effectively alleviate HaCaT cells apoptosis and overproduction of reactive oxygen species (ROS) induced by UVB. The results also showed that it inhibited the release of pro-inflammatory cytokines (IL-1α, IL-6 and IL-8); and suppressed the phosphorylation cascade of JNK and p38 MAPK to reduce the expression level of active-caspase3, ultimately prevented cell apoptosis. Thus, the EPS produced by VHPriobi O17 have the potential to be used for human anti-UVB irradiation.
本研究的目的是从乳酸菌（LAB）中分离胞外多糖（EPS），并评估EPS的抗紫外线B（UVB）活力。从34株乳酸菌中筛选出具有高EPS产量的VHPriobi O17。通过乙醇沉淀和DEAE-μSphere阴离子交换色谱法对VHPriobi O17产生的EPS（OP-2）进行纯化。通过离子色谱、傅里叶变换红外光谱（FT-IR）和凝胶柱色谱法分析，EPS（OP-2）是一种新型的类甘露糖多糖，重均分子量为84.2 kDa。EPS（OP-2）可有效减轻UVB诱导的HaCaT细胞凋亡和活性氧（ROS）的过量产生。结果还表明，它抑制促炎细胞因子（IL-1α、IL-6和IL-8）的释放；并抑制JNK和p38丝裂原活化蛋白激酶（MAPK）的磷酸化级联反应，以降低活性半胱天冬酶3的表达水平，最终防止细胞凋亡。因此，VHPriobi O17产生的EPS具有用于人类抗UVB辐射的潜力。
