VHPriobi O17产生的一种胞外多糖的分离、表征及抗紫外线辐射活性

Isolation, characterization and anti-UVB irradiation activity of an extracellular polysaccharide produced by VHPriobi O17.

作者信息

Peng Shudong, Guo Chaoqun, Wu Songjie, Duan Zhi

机构信息

School of Food Science and Engineering, South China University of Technology, Guangzhou, China.

Guangdong Youmei Institute of Intelligent Bio-Manufacturing, Foshan, 528225, China.

出版信息

Heliyon. 2022 Oct 17;8(10):e11125. doi: 10.1016/j.heliyon.2022.e11125. eCollection 2022 Oct.

The purpose of this study was to isolate exopolysaccharides (EPS) from lactic acid bacteria (LAB) and evaluate EPS anti-UVB viability. VHPriobi O17 with high EPS production was screened from 34 strains of LAB. The EPS (OP-2) produced by VHPriobi O17 was purified by alcohol precipitation and DEAE-μSphere anion exchange chromatography. By ion chromatography, FT-IR spectrum and gel column chromatography, EPS (OP-2) was a novel Man-like polysaccharide with the weight-averaged molecular of 84.2 kDa. The EPS (OP-2) can effectively alleviate HaCaT cells apoptosis and overproduction of reactive oxygen species (ROS) induced by UVB. The results also showed that it inhibited the release of pro-inflammatory cytokines (IL-1α, IL-6 and IL-8); and suppressed the phosphorylation cascade of JNK and p38 MAPK to reduce the expression level of active-caspase3, ultimately prevented cell apoptosis. Thus, the EPS produced by VHPriobi O17 have the potential to be used for human anti-UVB irradiation.

本研究的目的是从乳酸菌（LAB）中分离胞外多糖（EPS），并评估EPS的抗紫外线B（UVB）活力。从34株乳酸菌中筛选出具有高EPS产量的VHPriobi O17。通过乙醇沉淀和DEAE-μSphere阴离子交换色谱法对VHPriobi O17产生的EPS（OP-2）进行纯化。通过离子色谱、傅里叶变换红外光谱（FT-IR）和凝胶柱色谱法分析，EPS（OP-2）是一种新型的类甘露糖多糖，重均分子量为84.2 kDa。EPS（OP-2）可有效减轻UVB诱导的HaCaT细胞凋亡和活性氧（ROS）的过量产生。结果还表明，它抑制促炎细胞因子（IL-1α、IL-6和IL-8）的释放；并抑制JNK和p38丝裂原活化蛋白激酶（MAPK）的磷酸化级联反应，以降低活性半胱天冬酶3的表达水平，最终防止细胞凋亡。因此，VHPriobi O17产生的EPS具有用于人类抗UVB辐射的潜力。