Technical Vocational School, Department of Chemistry and Chemical Processing Technology, Aksaray University, Aksaray 68100, Turkey.
Department of Molecular Biology and Genetics, Faculty of Science and Letters, Aksaray University, Aksaray 68100, Turkey.
Int J Biol Macromol. 2024 Nov;280(Pt 4):136148. doi: 10.1016/j.ijbiomac.2024.136148. Epub 2024 Sep 30.
A novel structure of exopolysaccharide from the Lactic Acid Bacteria (LAB) Lacticaseibacillus rhamnosus ACS5, isolated from home-made Turkish cheese, is described. After lyophilization, the L-EPS-ACS5 was characterized in production and functional activities in vitro, including antioxidant and antidiabetic activities. The physicochemical characterizations of the L-EPS-ACS5 were determined through molecular weight, UV, FTIR, SEM, TGA, HPLC, NMR, methylation, and GC-MS analysis. Strong antioxidant activities of L-EPS-ACS5 were confirmed from the results obtained in the hydroxyl radical, DPPH, ABTS, FRAP, superoxide anion radical, total antioxidant activity, and DNA damage protective effect, and also the L-EPS-ACS5 exhibited high antidiabetic activity (60 %). This study isolated L-EPS-ACS5 from a home-made cheese L. rhamnosus strain, demonstrating its novel and enhanced functionalities compared to existing strains. This opens exciting avenues for its development in the fields of biomedicine and pharmaceuticals.
一种新型的乳酸菌（LAB）鼠李糖乳杆菌 ACS5 胞外多糖（EPS）结构，该菌株是从自制的土耳其奶酪中分离得到的。经冻干后，对 L-EPS-ACS5 的体外生产和功能活性进行了表征，包括抗氧化和抗糖尿病活性。通过分子量、紫外、傅里叶变换红外、扫描电子显微镜、热重分析、高效液相色谱、核磁共振、甲基化和气相色谱-质谱分析对 L-EPS-ACS5 的理化性质进行了测定。通过羟基自由基、DPPH、ABTS、FRAP、超氧阴离子自由基、总抗氧化活性和 DNA 损伤保护作用的结果，证实了 L-EPS-ACS5 具有很强的抗氧化活性，同时还具有高抗糖尿病活性（60%）。本研究从一种自制奶酪的 L. rhamnosus 菌株中分离出 L-EPS-ACS5，与现有菌株相比，它具有新颖且增强的功能。这为其在生物医学和制药领域的发展开辟了令人兴奋的途径。
