Hayat Khizar, Khan Jafar, Khan Asif, Ullah Shakir, Ali Shahid, Fu Yujie
Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, China.
Department of Botany, Abdul Wali Khan University, Mardan 23200, Pakistan.
Plants (Basel). 2021 Apr 19;10(4):796. doi: 10.3390/plants10040796.
Proline plays a significant role in the plant response to stress conditions. However, its role in alleviating metal-induced stresses remains elusive. We conducted an experiment to evaluate the ameliorative role of exogenous proline on cadmium-induced inhibitory effects in pigeon pea subjected to different Cd treatments (4 and 8 mg/mL). Cadmium treatments reduced photosynthetic attributes, decreased chlorophyll contents, disturbed nutrient uptake, and affected growth traits. The elevated activity of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase), in association with relatively high contents of hydrogen peroxide, thiobarbituric acid reactive substances, electrolyte leakage, and endogenous proline, was measured. Exogenous proline application (3 and 6 mM) alleviated cadmium-induced oxidative damage. Exogenous proline increased antioxidant enzyme activities and improved photosynthetic attributes, nutrient uptake (Mg, Ca, K), and growth parameters in cadmium-stressed pigeon pea plants. Our results reveal that proline supplementation can comprehensively alleviate the harmful effects of cadmium on pigeon pea plants.
脯氨酸在植物对胁迫条件的响应中发挥着重要作用。然而，其在减轻金属诱导的胁迫方面的作用仍不明确。我们进行了一项实验，以评估外源脯氨酸对不同镉处理（4和8毫克/毫升）下镉诱导的木豆抑制效应的改善作用。镉处理降低了光合特性，减少了叶绿素含量，扰乱了养分吸收，并影响了生长性状。测定了抗氧化酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）活性的升高，以及过氧化氢、硫代巴比妥酸反应性物质、电解质渗漏和内源脯氨酸的相对较高含量。外源脯氨酸处理（3和6毫摩尔）减轻了镉诱导的氧化损伤。外源脯氨酸提高了镉胁迫木豆植株的抗氧化酶活性，改善了光合特性、养分吸收（镁、钙、钾）和生长参数。我们的结果表明，脯氨酸补充可以全面减轻镉对木豆植株的有害影响。
