Iqbal Sajid, Qasim Muhammad, Rahman Hazir, Khan Naeem, Paracha Rehan Zafar, Bhatti Muhammad Faraz, Javed Aneela, Janjua Hussnain Ahmed
Department of Industrial Biotechnology, Atta-Ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12, Islamabad, Pakistan.
Department of Microbiology, Kohat University of Science and Technology (KUST), Kohat, Pakistan.
Mol Genet Genomics. 2023 Jan;298(1):79-93. doi: 10.1007/s00438-022-01964-5. Epub 2022 Oct 27.
Salinity severely affects crop yield by hindering nitrogen uptake and reducing plant growth. Plant growth-promoting bacteria (PGPB) are capable of providing cross-protection against biotic/abiotic stresses and facilitating plant growth. Genome-level knowledge of PGPB is necessary to translate the knowledge into a product as efficient biofertilizers and biocontrol agents. The current study aimed to isolate and characterize indigenous plant growth-promoting strains with the potential to promote plant growth under various stress conditions. In this regard, 72 bacterial strains were isolated from various saline-sodic soil/lakes; 19 exhibited multiple in vitro plant growth-promoting traits, including indole 3 acetic acid production, phosphate solubilization, siderophore synthesis, lytic enzymes production, biofilm formation, and antibacterial activities. To get an in-depth insight into genome composition and diversity, whole-genome sequence and genome mining of one promising Bacillus paralicheniformis strain ES-1 were performed. The strain ES-1 genome carries 12 biosynthetic gene clusters, at least six genomic islands, and four prophage regions. Genome mining identified plant growth-promoting conferring genes such as phosphate solubilization, nitrogen fixation, tryptophan production, siderophore, acetoin, butanediol, chitinase, hydrogen sulfate synthesis, chemotaxis, and motility. Comparative genome analysis indicates the region of genome plasticity which shapes the structure and function of B. paralicheniformis and plays a crucial role in habitat adaptation. The strain ES-1 has a relatively large accessory genome of 649 genes (~ 19%) and 180 unique genes. Overall, these results provide valuable insight into the bioactivity and genomic insight into B. paralicheniformis strain ES-1 with its potential use in sustainable agriculture.
盐度通过阻碍氮素吸收和抑制植物生长严重影响作物产量。植物促生细菌（PGPB）能够提供针对生物/非生物胁迫的交叉保护并促进植物生长。为了将PGPB的知识转化为高效生物肥料和生物防治剂产品，有必要了解其基因组水平的知识。本研究旨在分离和鉴定具有在各种胁迫条件下促进植物生长潜力的本土植物促生菌株。在这方面，从各种盐碱土壤/湖泊中分离出72株细菌菌株；其中19株表现出多种体外植物促生特性，包括吲哚-3-乙酸产生、磷溶解、铁载体合成、裂解酶产生、生物膜形成和抗菌活性。为了深入了解基因组组成和多样性，对一株有前景的解淀粉芽孢杆菌ES-1菌株进行了全基因组测序和基因组挖掘。ES-1菌株基因组携带12个生物合成基因簇、至少6个基因组岛和4个原噬菌体区域。基因组挖掘鉴定出了赋予植物促生能力的基因，如磷溶解、固氮、色氨酸产生、铁载体、3-羟基丁酮、丁二醇、几丁质酶、硫化氢合成、趋化性和运动性。比较基因组分析表明基因组可塑性区域塑造了解淀粉芽孢杆菌的结构和功能，并在栖息地适应中起关键作用。ES-1菌株有一个相对较大的辅助基因组，包含649个基因（约19%）和180个独特基因。总体而言，这些结果为解淀粉芽孢杆菌ES-1菌株的生物活性和基因组提供了有价值的见解，及其在可持续农业中的潜在用途。
