College of Clinical Medicine, Southwest Medical University, Luzhou, Sichuan, China.
Department of Pathology, the Affiliated Hospital of Southwest Medical University, No.25 Taiping Street, Jiangyang District, Luzhou, Sichuan, China.
Virchows Arch. 2022 Dec;481(6):847-852. doi: 10.1007/s00428-022-03439-9. Epub 2022 Oct 27.
Metastasis is the main cause of colorectal cancer (CRC)-related death and lymph node plays a vital role in this process. Long noncoding RNAs (lncRNAs) are emerging as an important factor of biological progress in cancers. However, lncRNAs related to CRC metastasis was rarely reported.CLAN expression data of tumor tissues and normal tissues were obtained from GEPIA database and 23 paired tumor and normal samples of patients. CLAN expression of 85 patients was carried out with RNA extracted from FFPE samples and quantified with qRT-PCR. Patients' clinical features were collected from department of Pathology of the Affiliated Hospital of Southwest Medical University. Immunohistochemistry staining was used to detect the metastasis-related proteins.CLAN was highly expressed in tumor tissues. And the expression level was not correlated with age, gender, differentiation, and location of CRC patients. Also, CLAN expression did not correlated with budding, LVI, and TILs. However, CLAN expression was strongly associated with lymph node metastasis and higher TNM stage. CLAN changed the lymphatic vessel density by promoting lymphangiogenesis but CLAN did not affect the blood vessel density.CLAN was a unique lncRNA that promoted lymphangiogenesis to accelerate CRC metastasis. CLAN might play a unique role in tumor early dissemination through lymphatic vessel.
转移是结直肠癌（CRC）相关死亡的主要原因，而淋巴结在这一过程中起着至关重要的作用。长链非编码 RNA（lncRNA）作为癌症生物学进展的一个重要因素正在兴起。然而，与 CRC 转移相关的 lncRNA 很少有报道。从 GEPIA 数据库中获取肿瘤组织和正常组织的 CLAN 表达数据，并对 85 例患者的肿瘤和正常组织进行 CLAN 表达检测，从 FFPE 样本中提取 RNA，并通过 qRT-PCR 进行定量。从西南医科大学附属医院病理科收集患者的临床特征。采用免疫组织化学染色检测转移相关蛋白。CLAN 在肿瘤组织中高表达。并且表达水平与 CRC 患者的年龄、性别、分化和部位无关。此外，CLAN 的表达与芽生、LVI 和 TILs 均无相关性。然而，CLAN 的表达与淋巴结转移和较高的 TNM 分期密切相关。CLAN 通过促进淋巴管生成改变淋巴管密度，但不影响血管密度。CLAN 是一种独特的 lncRNA，通过促进淋巴管生成来加速 CRC 的转移。CLAN 可能通过淋巴管在肿瘤早期播散中发挥独特作用。
