Department of Urology, Dankook University College of Medicine, Cheonan, Republic of Korea.
J Med Microbiol. 2022 Oct;71(10). doi: 10.1099/jmm.0.001585.
Antimicrobial resistance (AMR) among is a global issue. Understanding the transmission dynamics of infection is an important factor in reducing the occurrence of AMR. There is limited information on the genotyping and AMR traits of . Single-locus sequence-based (SLSB) sequence typing and genetic diversity analyses of AMR isolated from patients in the Republic of Korea were performed to clarify the transmission dynamics and eludicate proper management. Sanger sequencing of , 23S rRNA, and genes from a total of 103 . -positive specimens from 89 patients was carried out.. Twenty-seven different genotypes (GTs) were identified; 12 had been reported previously and 15 had not. GT7 and GT8 occurred frequently (=38, 36.89 %, and =16, 15.53 %, respectively). The genetic diversity of the AMR-determining sites was randomly dispersed among the different GTs. However, these GTs were classified into two phylogenetically distinct clusters that were significantly correlated with patient age and genetic diversity at positions 2058 and 2059 in the 23S rRNA gene. The GTs of 20 consecutive samples from 6 patients were compared to investigate temporal changes in GTs. One specimen changed its GT during follow-up, suggesting a new infection.. sequence typing can be a reliable tool for epidemiological studies. Two clusters have different characteristics in terms of genetic diversity. The cluster with genetic diversity in the AMR-determining site may be explained by the high prevalence of the specimens and subsequent antimicrobial exposure during the study period.
耐抗生素（AMR）是一个全球性的问题。了解感染的传播动态是减少 AMR 发生的一个重要因素。关于 分离株的基因分型和 AMR 特征的信息有限。对从韩国患者中分离的 AMR 进行单基因座序列基（SLSB） 序列分型和遗传多样性分析，以阐明传播动态并阐明适当的管理。对来自 89 名患者的总共 103 个 阳性标本的 23S rRNA 和 基因进行 Sanger 测序。鉴定了 27 种不同的 基因型（GTs）；其中 12 种先前有报道，15 种没有。GT7 和 GT8 频繁出现（=38，36.89%和=16，15.53%）。AMR 决定位点的遗传多样性在不同 GT 之间随机分散。然而，这些 GT 分为两个系统发育上明显不同的聚类，与患者年龄和 23S rRNA 基因中位置 2058 和 2059 的遗传多样性显著相关。对来自 6 名患者的 20 个连续样本的 GTs 进行比较，以调查 GTs 的时间变化。一个标本在随访过程中改变了其 GT，提示发生了新的感染。 序列分型可以成为流行病学研究的可靠工具。两个聚类在遗传多样性方面具有不同的特征。在 AMR 决定位点具有遗传多样性的聚类可能是由于研究期间标本的高流行率和随后的抗菌药物暴露所致。
