Chua Teck-Phui, Danielewski Jennifer A, Sweeney Emma L, Plummer Erica L, Bradshaw Catriona S, Whiley David M, Machalek Dorothy A, Garland Suzanne M, Murray Gerald L
Department of Obstetrics, Gynaecology and Newborn Health, University of Melbourne, Parkville, VIC, Australia.
Centre for Women's Infectious Diseases, The Royal Women's Hospital, Parkville, VIC, Australia.
Eur J Clin Microbiol Infect Dis. 2025 May;44(5):1167-1175. doi: 10.1007/s10096-025-05081-0. Epub 2025 Mar 3.
Molecular typing can identify relationships between M. genitalium strains and antimicrobial resistance and demographic data. We examined the association of mgpB sequence types (STs) with sex/sexual orientation, antimicrobial resistance and geographical location for M. genitalium in Australia.
Sequence data derived from previous studies in Victoria and Queensland were obtained from 170 M. genitalium samples for the mgpB, 23 S rRNA, and parC genes. An additional 55 M. genitalium samples from Victoria were sequenced for the same three genes in this study. A combined data set of 225 samples collected between 2017 and 2019 were examined for associations between mgpB ST and (i) sex/sexual orientation, (ii) macrolide and fluoroquinolone resistance, and (iii) geographical location using chi-square test.
Overall, 66 mgpB STs were identified; the most common were ST-7 (17.9%), ST-4 (11.6%), ST-105 (11.6%), and ST-2 (5.4%). There was a strong association between ST and sex/sexual orientation; ST-4 and ST-105 were most common among men-who-have-sex-with-men (p < 0.0001) while ST-7 among women (p < 0.0001). There was a strong association between ST and macrolide resistance (p = 0.0028). Fluoroquinolone resistance was less common (28.0%) and did not differ by STs (p = 0.20). There was no association between ST and geographic location (p = 0.056).
In this Australian study, four mgpB STs were common and were strongly associated with sex/sexual orientation and macrolide resistance. This relationship was not seen for fluoroquinolone resistance nor geographic location. These findings highlight the sporadic nature of resistance, indicating a need for effective treatment approaches combined with routine antimicrobial resistance surveillance.
分子分型可确定生殖支原体菌株之间的关系以及抗菌药物耐药性和人口统计学数据。我们研究了澳大利亚生殖支原体mgpB序列类型（STs）与性别/性取向、抗菌药物耐药性和地理位置之间的关联。
从之前在维多利亚州和昆士兰州的研究中获取了170份生殖支原体样本的mgpB、23S rRNA和parC基因的序列数据。本研究中对另外55份来自维多利亚州的生殖支原体样本进行了相同三个基因的测序。使用卡方检验，对2017年至2019年期间收集的225份样本的合并数据集进行mgpB ST与（i）性别/性取向、（ii）大环内酯类和氟喹诺酮类耐药性以及（iii）地理位置之间关联的研究。
总体而言，共鉴定出66种mgpB STs；最常见的是ST-7（17.9%）、ST-4（11.6%）、ST-105（11.6%）和ST-2（5.4%）。ST与性别/性取向之间存在强关联；ST-4和ST-105在男男性行为者中最为常见（p<0.0001），而ST-7在女性中最为常见（p<0.0001）。ST与大环内酯类耐药性之间存在强关联（p=0.0028）。氟喹诺酮类耐药性不太常见（28.0%），且在不同STs之间无差异（p=0.20）。ST与地理位置之间无关联（p=0.056）。
在这项澳大利亚研究中，四种mgpB STs较为常见，且与性别/性取向和大环内酯类耐药性密切相关。氟喹诺酮类耐药性和地理位置方面未观察到这种关系。这些发现突出了耐药性的散发性，表明需要有效的治疗方法并结合常规的抗菌药物耐药性监测。
