Usman Ibe Michael, Adebisi Samuel Sunday, Musa Sunday Abraham, Iliya Ibrahim Abdullahi, Ochieng Juma John, Ivang Andrew Ekpeyong, Peter Akwu Bala, Okesina Akeem Ayodeji
Department of Human Anatomy, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
Department of Human Anatomy, Kampala International University, Bushenyi, Uganda.
J Exp Pharmacol. 2022 Oct 20;14:275-289. doi: 10.2147/JEP.S369631. eCollection 2022.
The recent increase in aluminum exposure and its effect on the development of the brain call for serious attention. The study investigated the behavioral and immunohistochemical changes in the cerebral cortex of Wistar rats following prenatal co-administration of ethyl acetate leaf fraction of (EATI) and aluminum chloride (AlCl).
Pregnant Wistar rats were divided into 5 groups (n=4). Group I (negative control), Group II-V were experimental groups treated with 200 mg/kg of AlCl s/c. Group III and IV received an additional 400 mg/kg and 800 mg/kg of EATI respectively, while Group V received an additional 300 mg/kg of Vitamin E for 14 days (prenatal days 7-21) via the oral route. The pups were then exposed to cliff avoidance, negative geotaxis, and elevated plus maze (EPM) test on the post-natal day (PoND) 4-6, 7-10, and 18 respectively. On PoND 21 pups were sacrificed, and the skull dissected to remove the brain. The harvested brain tissues were processed for Cresyl fast (CF) and glial fibrillary acid protein (GFAP).
The study showed that EATI administration during AlCl exposure was associated with significant improvement in sensory-motor development. The EPM, CF, and GFAP results revealed significant improvement in anxiety-like behavior, motor activities, GFAP expression, pyramidal cell count, and Nissl staining following prenatal EATI administration during AlCl exposure.
The present study concludes that EATI was associated with some protective potential during prenatal AlCl exposure in Wistar rats.
近期铝暴露的增加及其对大脑发育的影响值得严重关注。本研究调查了在孕期共同给予（EATI）乙酸乙酯叶提取物和氯化铝（AlCl）后，Wistar大鼠大脑皮质的行为和免疫组化变化。
将怀孕的Wistar大鼠分为5组（n = 4）。第一组（阴性对照组），第二组至第五组为实验组，皮下注射200 mg/kg的AlCl。第三组和第四组分别额外给予400 mg/kg和800 mg/kg的EATI，而第五组通过口服途径在14天内（孕期第7 - 21天）额外给予300 mg/kg的维生素E。然后分别在出生后第4 - 6天、第7 - 10天和第18天对幼崽进行避崖试验、负趋地性试验和高架十字迷宫（EPM）试验。在出生后第21天处死幼崽，解剖颅骨取出大脑。对收获的脑组织进行甲酚固绿（CF）和胶质纤维酸性蛋白（GFAP）处理。
研究表明，在AlCl暴露期间给予EATI与感觉运动发育的显著改善有关。EPM、CF和GFAP结果显示，在AlCl暴露期间孕期给予EATI后，焦虑样行为、运动活动、GFAP表达、锥体细胞计数和尼氏染色有显著改善。
本研究得出结论，在Wistar大鼠孕期AlCl暴露期间，EATI具有一定的保护潜力。
