Mignucci-Giannoni Antonio A, Alsina-Guerrero Mayela M
Caribbean Manatee Conservation Center, Inter American University of Puerto Rico, Bayamón, PR, USA.
Center for Conservation Medicine and Ecosystem Health, Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis.
Vet Med Int. 2022 Oct 18;2022:4305838. doi: 10.1155/2022/4305838. eCollection 2022.
Antillean manatees () are endangered throughout the southwestern Gulf of Mexico, the Caribbean coast of Central and South America, the Greater Antilles, and the northeastern coast of South America to Brazil. Establishing blood reference intervals is essential as a tool in classifying health status, diagnosing, establishing treatment regimens, and monitoring the progress of a disease in rescued manatees. We collected blood samples from 44 free-ranging and 26 rescued manatees from Puerto Rico between 1992 and 2020 for hematology and blood chemistry analysis. We obtained values for white blood cell count and red blood cell count, hemoglobin, hematocrit, platelet, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration, and red cell distribution width. A manual leukocyte differential allowed for the evaluation of different cell types. In addition, we performed a comprehensive metabolic panel on serum samples. These analytes were grouped based on six physiologic processes: liver-associated enzymes and pigments; muscle-associated enzymes; kidney-associated compounds and products; sugars, lipids, and pancreatic-associated enzymes; proteins; and electrolytes. For every parameter, summary statistics of values were calculated on all the samples. Reference ranges were determined as ±1 standard deviation around the mean. An unpaired two-sample -test was done comparing males versus females and adults versus calves for any significant differences ( ≤ 0.05). We establish the reference intervals of hematology and blood chemistry for the population of Antillean manatees in Puerto Rico and compare them with those established for manatees from Belize, Brazil, Florida, Guyana, and Mexico.
安的列斯海牛（）在墨西哥湾西南部、中美洲和南美洲的加勒比海岸、大安的列斯群岛以及从南美洲东北海岸到巴西的区域均面临濒危状况。建立血液参考区间作为一种工具，对于分类获救海牛的健康状况、进行诊断、制定治疗方案以及监测疾病进展至关重要。1992年至2020年间，我们从波多黎各的44头自由放养海牛和26头获救海牛身上采集了血液样本，用于血液学和血液化学分析。我们获得了白细胞计数、红细胞计数、血红蛋白、血细胞比容、血小板、平均红细胞体积、平均红细胞血红蛋白、平均红细胞血红蛋白浓度和红细胞分布宽度的值。通过手工白细胞分类来评估不同的细胞类型。此外，我们对血清样本进行了全面的代谢检测。这些分析物根据六个生理过程进行分组：肝脏相关酶和色素；肌肉相关酶；肾脏相关化合物和产物；糖、脂质和胰腺相关酶；蛋白质；以及电解质。对于每个参数，计算了所有样本值的汇总统计量。参考范围确定为均值±1个标准差。进行了非配对双样本检验，比较雄性与雌性以及成年海牛与幼崽之间是否存在显著差异（≤0.05）。我们确定了波多黎各安的列斯海牛种群的血液学和血液化学参考区间，并将它们与为来自伯利兹、巴西、佛罗里达、圭亚那和墨西哥的海牛所建立的参考区间进行比较。
