Cheu Ryan K, Mohammadi Avid, Schifanella Luca, Broedlow Courtney, Driscoll Connor B, Miller Charlene J, Reeves R Keith, Yudin Mark H, Hensley-McBain Tiffany, Kaul Rupert, Klatt Nichole R
Department of Pharmaceutics, University of Washington, Seattle, WA, United States.
Washington National Primate Research Center, University of Washington, Seattle, WA, United States.
Front Reprod Health. 2022 Jul 13;4:876729. doi: 10.3389/frph.2022.876729. eCollection 2022.
The role of neutrophils relative to vaginal dysbiosis is unclear. We hypothesize that bacterial vaginosis (BV)-associated bacteria may induce the activation and accumulation of mucosal neutrophils within the female reproductive tract (FRT), resulting in epithelial barrier damage. We collected endocervical cytobrushes from women with and without BV and assessed bacteria community type and frequency/functional phenotypes of neutrophils. We performed whole blood co-cultures with BV-associated bacteria and healthy vaginal commensals and assessed their impact on epithelial integrity using transepithelial electrical resistance. We demonstrated increased neutrophil frequency ( < 0.0001), activation ( < 0.0001), and prolonged lifespan ( < 0.0001) in the cytobrushes from women with non- dominant (nLD) communities. Our co-cultures confirmed these results and identified significant barrier damage in the presence of neutrophils and . Here, we demonstrate that BV-associated bacteria induce neutrophil activation and increase lifespan, potentially causing accumulation in the FRT and epithelial barrier damage.
中性粒细胞在阴道生态失调中的作用尚不清楚。我们推测，细菌性阴道病（BV）相关细菌可能会诱导女性生殖道（FRT）内黏膜中性粒细胞的激活和聚集，从而导致上皮屏障受损。我们收集了患有和未患有BV的女性的宫颈内膜细胞刷，并评估了细菌群落类型以及中性粒细胞的频率/功能表型。我们将全血与BV相关细菌和健康的阴道共生菌进行共培养，并使用跨上皮电阻评估它们对上皮完整性的影响。我们证明，在具有非优势（nLD）群落的女性的细胞刷中，中性粒细胞频率增加（<0.0001）、激活增加（<0.0001）且寿命延长（<0.0001）。我们的共培养证实了这些结果，并确定在存在中性粒细胞的情况下存在明显的屏障损伤。在此，我们证明BV相关细菌会诱导中性粒细胞激活并延长其寿命，可能导致其在FRT中积聚并造成上皮屏障损伤。
