Caixeta Magalhães Tibúrcio Aline Andréia, Paiva Aline Dias, Pedrosa André Luiz, Rodrigues Wellington Francisco, Bernardes da Silva Raíssa, Oliveira Adriana Gonçalves
Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.
Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.
Heliyon. 2022 Oct 18;8(10):e11154. doi: 10.1016/j.heliyon.2022.e11154. eCollection 2022 Oct.
Biofilm formation is a key factor in the pathogenesis of enterococcal infections. Thus, the biofilm-forming ability and frequency of biofilm-related genes in (PRASEF) compared to penicillin- and ampicillin-susceptible (PSASEF) were assessed in the present study. In addition, the effect of sub-inhibitory concentrations (sub-MICs) of antibiotics on biofilm formation and expression of virulence genes was evaluated. Twenty PRASEF and 21 PSASEF clinical isolates were used to determine the effect of sub-MICs of antibiotics (ampicillin, penicillin, and gentamicin) on biofilm formation, and ten selected isolates were subjected to RT-qPCR to detect the transcript levels of virulence genes (, , , and ). Antibiotic susceptibility was evaluated by the microdilution broth method. Biofilm formation assay was performed using the microtiter plate method. All PSASEF and PRASEF isolates produced biofilms . Most isolates had three or four virulence genes. Sub-MICs of ampicillin significantly decreased biofilm production and expression of and genes, although the transcript levels were significantly lower (-350% and -606.2%, respectively) among the PSASEF isolates only. Sub-MICs of gentamicin did not have any significant effect on biofilm formation, but slightly increased the transcript levels of . In conclusion, this study showed that the biofilm-forming ability and frequency of the evaluated virulence genes were similar among the PRASEF and PSASEF isolates. Further, antibiotic sub-MICs were confirmed to interfere with the expression pattern of virulence genes and biofilm formation by . However, further studies are required to clarify the role of sublethal doses of antibiotics on enterococcal biofilms.
生物膜形成是肠球菌感染发病机制中的一个关键因素。因此，在本研究中评估了耐青霉素和氨苄西林肠球菌（PRASEF）与青霉素和氨苄西林敏感肠球菌（PSASEF）的生物膜形成能力及生物膜相关基因的出现频率。此外，还评估了抗生素亚抑菌浓度（亚MICs）对生物膜形成和毒力基因表达的影响。使用20株PRASEF和21株PSASEF临床分离株来确定抗生素（氨苄西林、青霉素和庆大霉素）亚MICs对生物膜形成的影响，并对10株选定的分离株进行RT-qPCR以检测毒力基因（、、和）的转录水平。通过微量肉汤稀释法评估抗生素敏感性。使用微量滴定板法进行生物膜形成测定。所有PSASEF和PRASEF分离株均形成生物膜。大多数分离株有三到四个毒力基因。氨苄西林亚MICs显著降低了生物膜产生以及和基因的表达，不过仅在PSASEF分离株中，转录水平显著更低（分别降低-350%和-606.2%）。庆大霉素亚MICs对生物膜形成没有任何显著影响，但略微增加了的转录水平。总之，本研究表明，PRASEF和PSASEF分离株的生物膜形成能力及所评估毒力基因的出现频率相似。此外，已证实抗生素亚MICs会干扰毒力基因的表达模式以及由形成的生物膜。然而，需要进一步研究来阐明亚致死剂量抗生素在肠球菌生物膜中的作用。
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