Department of Gastrointestinal Surgery, Jiangmen Central Hospital, Jiangmen, China.
The First Affiliated Hospital, Jinan University, Guangzhou, China.
Front Immunol. 2022 Oct 11;13:1043512. doi: 10.3389/fimmu.2022.1043512. eCollection 2022.
CUL7, a gene composed of 26 exons associated with cullin 7 protein, is also an E3 ligase that is closely related to cell senescence, apoptosis, and cell transformation and also plays an important role in human cancer. However, there is no systematic pan-cancer analysis has been performed to explore its role in prognosis and immune prediction. In this study, the expression of CUL7 in colon adenocarcinoma (COAD) was investigated to determine its prognosis value. First, based on the Cancer Genome Atlas (TCGA), Genotypic-Tissue Expression Project(GTEx), Cancer Cell Line Encyclopedias(CCLE), and TISIDB database, the potential role of CUL7 in different tumors was explored. Subsequently, the expression of CUL7 in COAD was explored and verified by Immunohistochemistry (IHC). Furthermore, the mutation frequency of CUL7 in COAD was analyzed, and the prognostic value of CUL7 in COAD was discussed. In addition, the nomogram was constructed, and its prognostic value was verified by follow-up data from Jiangmen Central Hospital. Finally, PPI network analysis explored the potential biological function of CUL7 in COAD. The results show that CUL7 is upregulated in most tumors, which is significantly associated with poor survival. At the same time, CUL7 is correlated with the clinical stage and immune landscape of various tumors. In colorectal cancer, CUL7 was overexpressed in tumor tissues by IHC with a mutation frequency of about 4%. CUL7 is an independent prognostic factor for colorectal cancer. The nomogram constructed has effective predictive performance, and external databases proved the prognostic value of CUL7. In addition, PPI network analysis showed that CUL7 was closely related to FBXW8, and further pathway enrichment analysis showed that CUL7 was mainly involved in ubiquitin-mediated proteolysis. Therefore, our study provides a comprehensive understanding of the potential role of CUL7 in different tumors, and CUL7 might be a prognostic marker for COAD.
CUL7 是一个由 26 个外显子组成的基因，与 CUL7 蛋白有关，也是一种 E3 连接酶，与细胞衰老、凋亡和细胞转化密切相关，在人类癌症中也发挥着重要作用。然而，目前还没有系统的泛癌分析来探讨其在预后和免疫预测中的作用。在这项研究中，我们研究了结肠腺癌（COAD）中 CUL7 的表达，以确定其预后价值。首先，基于癌症基因组图谱（TCGA）、基因型组织表达计划（GTEx）、癌症细胞系百科全书（CCLE）和 TISIDB 数据库，探讨了 CUL7 在不同肿瘤中的潜在作用。随后，通过免疫组织化学（IHC）探讨和验证了 CUL7 在 COAD 中的表达。此外，分析了 CUL7 在 COAD 中的突变频率，并讨论了 CUL7 在 COAD 中的预后价值。此外，构建了列线图，并通过江门中心医院的随访数据验证了其预后价值。最后，通过 PPI 网络分析探讨了 CUL7 在 COAD 中的潜在生物学功能。结果表明，CUL7 在大多数肿瘤中上调，与生存不良显著相关。同时，CUL7 与各种肿瘤的临床分期和免疫图谱相关。在结直肠癌中，CUL7 通过 IHC 在肿瘤组织中高表达，突变频率约为 4%。CUL7 是结直肠癌的独立预后因素。构建的列线图具有有效的预测性能，外部数据库证明了 CUL7 的预后价值。此外，PPI 网络分析表明 CUL7 与 FBXW8 密切相关，进一步的通路富集分析表明 CUL7 主要参与泛素介导的蛋白水解。因此，我们的研究提供了对 CUL7 在不同肿瘤中潜在作用的全面了解，CUL7 可能是 COAD 的预后标志物。
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