Institute of Microbiology and Genetics, Georg August University Göttingen, Göttingen, Germany.
Methods Mol Biol. 2023;2555:13-21. doi: 10.1007/978-1-0716-2795-2_2.
Microbial communities play an important role in marine ecosystem processes. Although the number of studies targeting marker genes such as the 16S rRNA gene has increased during the last years, the vast majority of marine diversity are rather unexplored. Moreover, most studies focused on the entire microbial community and thus do not assess the active fraction of the microbial community. Here, we describe a detailed protocol for the simultaneous extraction of DNA and RNA from marine water samples and the generation of cDNA from the isolated RNA that can be used as a universal template in various marker gene studies.
微生物群落在海洋生态系统过程中发挥着重要作用。尽管近年来针对 16S rRNA 基因等标记基因的研究数量有所增加，但绝大多数海洋生物多样性仍未得到充分探索。此外，大多数研究都集中在整个微生物群落上，因此无法评估微生物群落的活性部分。在这里，我们描述了一种从海洋水样中同时提取 DNA 和 RNA 并从分离的 RNA 生成 cDNA 的详细方案，该 cDNA 可用作各种标记基因研究中的通用模板。
