ICP, CSIC, Madrid, Spain.
Centre for Environmental Biotechnology, School of Natural Sciences, Bangor University, Bangor, United Kingdom.
Methods Mol Biol. 2023;2555:181-194. doi: 10.1007/978-1-0716-2795-2_13.
The discovery of new enzymes is strongly enabled by the implementation of high-throughput screening methods to detect enzymatic activity in single organisms or clone expression libraries, or to benchmark their performances against known prototypes. In this chapter, a number of methods, applicable at high-throughput scale, are described that allow the screening and characterization of enzymes relevant to biotechnology, particularly, ester-hydrolases (esterases, lipases, phospholipases, and polyester hydrolases).
新酶的发现得益于高通量筛选方法的实施，这些方法可用于检测单个生物体或克隆表达文库中的酶活性，或针对已知原型对其性能进行基准测试。在本章中，描述了一些适用于高通量规模的方法，这些方法可用于筛选和表征与生物技术相关的酶，特别是酯水解酶（酯酶、脂肪酶、磷脂酶和聚酯水解酶）。
