妊娠特异性β-1 糖蛋白 1 高表达与胃癌预后不良相关。
High Expression of Pregnancy Specific Beta-1-glycoprotein 1 Is Associated With Poor Gastric Cancer Prognosis.
机构信息
Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-associated Disorder Control Technology, Chosun University, Gwangju, Republic of Korea.
Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-associated Disorder Control Technology, Chosun University, Gwangju, Republic of Korea;
出版信息
In Vivo. 2022 Nov-Dec;36(6):2700-2707. doi: 10.21873/invivo.13005.
BACKGROUND/AIM: Pregnancy specific beta-1-glycoprotein 1 (PSG1) is a member of the immunoglobulin superfamily and associated with carcinoembryonic antigens. It has been reported to be highly expressed in variety of cancers. However, the role of PSG1 in gastric cancer remains unclear. The aim of our study was to examine the clinical significance and functional role of PSG1 in gastric cancer.
MATERIALS AND METHODS
We analyzed the association between PSG1 expression levels and clinicopathological features using Kaplan-Meier survival curves and publicly available microarray data. In gastric cancer cell lines, PSG1 expression levels were detected by polymerase chain reaction and western blot analysis. The functional role of PSG1 on the proliferation, migration and invasive abilities were also investigated using PSG1 siRNA or an over-expression plasmid vector through WST, transwell migration and invasion assays.
RESULTS
PSG1 expression levels were higher in gastric cancer patient tissues than in normal gastric tissues. Increased expression of PSG1 was associated with poor patient prognosis. Knockdown of PSG1 inhibited cell proliferation, migration, and invasion in gastric cancer cells. In contrast, over-expression of PSG1 enhanced cell proliferation, migration, and invasion.
CONCLUSION
PSG1 is up-regulated in gastric cancer and may serve as an oncogene that promotes cell proliferation, migration, and invasion. PSG1 is an independent prognostic factor for the progression of gastric cancer and may be a potential biomarker and therapeutic target for gastric cancer.
背景/目的：妊娠特异性β-1-糖蛋白 1（PSG1）是免疫球蛋白超家族的成员，与癌胚抗原相关。据报道，它在多种癌症中高度表达。然而，PSG1 在胃癌中的作用尚不清楚。我们的研究旨在探讨 PSG1 在胃癌中的临床意义和功能作用。
材料和方法
我们使用 Kaplan-Meier 生存曲线和公开的微阵列数据分析 PSG1 表达水平与临床病理特征之间的关联。在胃癌细胞系中，通过聚合酶链反应和 Western blot 分析检测 PSG1 表达水平。通过 PSG1 siRNA 或过表达质粒载体通过 WST、Transwell 迁移和侵袭实验研究 PSG1 对增殖、迁移和侵袭能力的功能作用。
结果
PSG1 表达水平在胃癌患者组织中高于正常胃组织。PSG1 表达增加与患者预后不良相关。PSG1 的敲低抑制了胃癌细胞的增殖、迁移和侵袭。相比之下，PSG1 的过表达增强了细胞的增殖、迁移和侵袭。
结论
PSG1 在胃癌中上调，可能作为促进细胞增殖、迁移和侵袭的癌基因。PSG1 是胃癌进展的独立预后因素，可能是胃癌的潜在生物标志物和治疗靶点。
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