Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, PR China.
Ministry of Agriculture Key Laboratory of Frozen Prepared Marine Foods Processing, Taixiang Group, Rongcheng Taixiang Food Products Co., Ltd, PR China.
Food Microbiol. 2023 Feb;109:104151. doi: 10.1016/j.fm.2022.104151. Epub 2022 Sep 23.
We investigated the function of pyoverdine in the biofilm formation, motility, and spoilage potential of Pseudomonas fluorescens. We targeted and identified two major genes (pvdA and pvdE) that are involved in the biosynthesis of siderophores. We next constructed ΔpvdA and ΔpvdE mutants of P. fluorescens PF08 and found that the deletion of pyoverdine led to a biofilm-to-motivity transition as both ΔpvdA and ΔpvdE mutants displayed enhanced motility, reduced level of exopolysaccharides (EPSs), and attenuated biofilm formation. In addition, the lack of synthesis of pyoverdine promoted the spoilage of fish fillets stored at 4 °C. Based on the effect of pyoverdine deletion on the phenotype; we report that pyoverdine regulates the transcription levels of htpX, phoA, flip, flgA, and RpoS, suggesting that pyoverdine-mediated iron absorption may affect the regulation of flagellum and stress resistance. This study emphasizes the important role of pyoverdine in the formation of biofilm, motility, and spoilage of P. fluorescens PF08.
我们研究了绿脓假单胞菌中吡咯并喹啉啉酮（pyoverdine）在生物膜形成、运动性和腐败潜能中的功能。我们针对并鉴定了两个参与铁载体生物合成的主要基因（pvdA 和 pvdE）。接下来，我们构建了绿脓假单胞菌 PF08 的ΔpvdA 和ΔpvdE 突变体，发现缺失吡咯并喹啉啉酮导致生物膜到运动性的转变，因为ΔpvdA 和ΔpvdE 突变体均表现出增强的运动性、降低的胞外多糖（EPS）水平和减弱的生物膜形成。此外，缺乏吡咯并喹啉啉酮的合成促进了 4°C 下鱼片的腐败。基于吡咯并喹啉啉酮缺失对表型的影响，我们报告说吡咯并喹啉啉酮调节 htpX、phoA、flip、flgA 和 RpoS 的转录水平，表明吡咯并喹啉啉酮介导的铁吸收可能影响鞭毛和应激抗性的调节。本研究强调了吡咯并喹啉啉酮在绿脓假单胞菌 PF08 生物膜形成、运动性和腐败中的重要作用。
