Meyer Jean-Marie, Stintzi Alain, Coulanges Valie, Shivaji Sisinthy, Voss Jessica A, Taraz Kambiz, Budzikiewic Herbert
Laboratoire de Microbiologie et de Gbnbtique, Universite Louis-Pasteur, UPRES A du CNRS no. 7010,28 rue Goethe, 67000 Strasbourg, France.
Centre for Cellular and Molecular Biology, Hyderabad 500 007, India.
Microbiology (Reading). 1998 Nov;144 ( Pt 11):3119-3126. doi: 10.1099/00221287-144-11-3119.
Five independent fluorescent pseudomonad isolates originating from Antarctica were analysed for their pyoverdine systems. A pyoverdine-related siderotyping, which involved pyoverdine-induced growth stimulation, pyoverdine-mediated iron uptake, pyoverdine analysis by electrophoresis and isoelectric focusing, revealed three different pyoverdine-related siderotypes among the five isolates. One siderotype, including Pseudomonas fluorescens 1W and P. fluorescens 10CW, was identical to that of P. fluorescens ATCC 13525. Two other strains, P. fluorescens 9AW and Pseudomonas putida 9BW, showed identical pyoverdine-related behaviour to each other, whereas the fifth strain, P. fluorescens 51W, had unique features compared to the other strains or to a set of 12 fluorescent Pseudomonas strains used as comparison material. Elucidation of the structure of the pyoverdines produced by the Antarctic strains supported the accuracy of the siderotyping methodology by confirming that pyoverdines from strains 1W and 10CW had the same structures as the P. fluorescens ATCC 13525 pyoverdine, whereas the 9AW and 9BW pyoverdines are probably identical with the pyoverdine of P. fluorescens strain 244. Pyoverdine from strain 51W appeared to be a novel pyoverdine since its structure was different from all previously established pyoverdine structures. Together with the conclusion that the Antarctic Pseudomonas strains have no special features at the level of their pyoverdines and pyoverdine-mediated iron metabolism compared to worldwide strains, the present work demonstrates that siderotyping provides a rapid means of screening for novel pyoverdines.
对五株源自南极洲的独立荧光假单胞菌分离株的铁载体系统进行了分析。一种与铁载体相关的铁载体分型方法，包括铁载体诱导的生长刺激、铁载体介导的铁摄取、通过电泳和等电聚焦对铁载体进行分析，结果显示这五株分离株中有三种不同的与铁载体相关的铁载体型。一种铁载体型，包括荧光假单胞菌1W和荧光假单胞菌10CW，与荧光假单胞菌ATCC 13525的铁载体型相同。另外两株菌株，荧光假单胞菌9AW和恶臭假单胞菌9BW，彼此表现出相同的与铁载体相关的行为，而第五株菌株荧光假单胞菌51W与其他菌株或用作比较材料的一组12株荧光假单胞菌菌株相比具有独特的特征。对南极菌株产生的铁载体结构的阐明，通过确认1W和10CW菌株的铁载体与荧光假单胞菌ATCC 13525的铁载体具有相同的结构，支持了铁载体分型方法的准确性，而9AW和9BW的铁载体可能与荧光假单胞菌244菌株的铁载体相同。51W菌株的铁载体似乎是一种新型铁载体，因为其结构与所有先前确定的铁载体结构不同。与南极假单胞菌菌株在其铁载体和铁载体介导的铁代谢水平上与全球菌株相比没有特殊特征的结论一起，本研究表明铁载体分型提供了一种筛选新型铁载体的快速方法。
