Department of Integrated Biomedical and Life Sciences, Graduate School, Korea University.
BK21FOUR R&E Center for Learning Health Systems, Korea University.
J Oleo Sci. 2022;71(11):1679-1688. doi: 10.5650/jos.ess22215.
Stearidonic acid (SDA) is a plant-based n-3 polyunsaturated fatty acid with multiple biological activities. The enrichment of SDA and synthesis of triacylglycerol (TAG) were carried out consecutively via two lipase-catalyzed reactions, hydrolysis, and esterification. First, SDA was enriched into a glyceride fraction from ahiflower seed oil by Candida rugosa lipase-catalyzed hydrolysis. Under the optimum conditions of 35°C, 0.1% lipase powder of Lipase OF, and 50% buffer solution (based on the weight of total substrate), SDA was enriched from 21.6 to 40.7 wt% in glyceride fraction. SDA-enriched TAG was then synthesized from the SDA-enriched glyceride and the SDA-enriched fatty acid via esterification using an in-house immobilized lipase as a biocatalyst. The SDA-enriched fatty acid was obtained from part of the SDA-enriched glyceride by saponification and the in-house immobilized lipase was prepared from Eversa Transform 2.0 using Lewatit VP OC 1600 as a carrier. The optimum reaction conditions for the synthesis of TAG were a temperature of 50°C, an enzyme loading of 10%, and a vacuum of 10 mmHg. A maximum conversion to TAG of ca. 94% was achieved after 12 h under the optimum conditions.
硬脂烯酸（SDA）是一种植物来源的 n-3 多不饱和脂肪酸，具有多种生物学活性。通过两步脂肪酶催化反应，即水解和酯化，连续进行 SDA 的富集和三酰基甘油（TAG）的合成。首先，通过 Candida rugosa 脂肪酶催化水解，将 SDA 从印加果籽油中富集到甘油酯馏分中。在 35°C、0.1%脂肪酶粉末 Lipase OF 和 50%缓冲溶液（基于总底物重量）的最佳条件下，SDA 在甘油酯馏分中的含量从 21.6%富集到 40.7wt%。然后，通过酯化反应，使用固定化脂肪酶作为生物催化剂，从 SDA 富集的甘油酯和 SDA 富集的脂肪酸合成 SDA 富集的 TAG。SDA 富集的脂肪酸是通过部分 SDA 富集的甘油酯皂化获得的，固定化脂肪酶是使用 Lewatit VP OC 1600 作为载体从 Eversa Transform 2.0 中制备的。TAG 合成的最佳反应条件为温度 50°C、酶用量 10%和真空度 10mmHg。在最佳条件下反应 12 小时后，TAG 的转化率最高可达约 94%。
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