Okamoto Satoru, Kawasaki Azusa
Graduate School of Science and Technology, Niigata University, Niigata, Japan.
PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan.
Bio Protoc. 2022 Oct 5;12(19). doi: 10.21769/BioProtoc.4520.
A number of molecules, such as secreted peptides, have been shown to mediate root-to-shoot signaling in response to various conditions. The xylem is a pathway for water and molecules that are translocated from roots to shoots. Therefore, collecting and analyzing xylem exudates is an efficient approach to study root-to-shoot long-distance signaling. Here, we describe a step-by-step protocol for the collection of xylem exudate from the model plant and the crop plant soybean ( ). In this protocol, we can collect xylem exudate from plants cultured under normal growth conditions without using special equipment. Graphical abstract: Xylem exudates on the cut surfaces of an hypocotyl and a soybean internode.
许多分子，如分泌肽，已被证明在响应各种条件时介导根到地上部的信号传导。木质部是水分和从根部转运到地上部的分子的通道。因此，收集和分析木质部渗出液是研究根到地上部长距离信号传导的有效方法。在这里，我们描述了一种从模式植物和作物大豆（ ）中收集木质部渗出液的分步方案。在本方案中，我们可以在不使用特殊设备的情况下，从正常生长条件下培养的植物中收集木质部渗出液。图形摘要：拟南芥下胚轴和大豆节间切面上的木质部渗出液。
