使用组织透明化和点击化学技术，以细胞分辨率可视化哺乳动物组织中靶标特异性药物结合的方案。
A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry.
机构信息
Department of Neuroscience, The Scripps Research Institute, La Jolla, CA 92037, USA.
Department of Neuroscience, The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
出版信息
STAR Protoc. 2022 Oct 25;3(4):101778. doi: 10.1016/j.xpro.2022.101778. eCollection 2022 Dec 16.
Here, we provide a protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution. By combining tissue clearing and click chemistry, this protocol allows fluorescence tagging of covalent drug binding . In addition, the protocol is compatible with molecular marker staining for cell type identifications. For complete details on the use and execution of this protocol, please refer to Pang et al. (2022).
在这里，我们提供了一个方案，可在具有细胞分辨率的哺乳动物组织中可视化靶标特异性药物结合。通过结合组织透明化和点击化学，该方案允许共价药物结合的荧光标记。此外，该方案与分子标记物染色兼容，可用于细胞类型鉴定。有关此方案使用和执行的完整详细信息，请参阅 Pang 等人（2022 年）。
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