Medical Scientist Training Program, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Division of Experimental Pathology, Department of Pathology, UPMC, Pittsburgh, PA 15213, USA.
STAR Protoc. 2022 Feb 15;3(1):101178. doi: 10.1016/j.xpro.2022.101178. eCollection 2022 Mar 18.
Although there are numerous tissue clearing protocols, most are inadequate for clearing liver tissue. Here we present a flexible protocol for mouse liver tissue; we combine strategies from several previously published protocols for delipidation, decolorization, staining, and refractive index matching. LiverClear is sufficiently versatile to allow clearing of healthy and diseased mouse liver followed by immunofluorescence staining and imaging to visualize intact 3D structures such as bile ducts and hepatocyte canaliculi. We also adapted this protocol for clearing human livers. For complete details on the use and execution of this protocol, please refer to Molina et al. (2021).
尽管有许多组织透明化方案，但大多数都不适合肝脏组织的透明化。在这里，我们提出了一个适用于小鼠肝脏组织的灵活方案；我们结合了之前发表的几个方案中的策略，用于去脂、脱色、染色和折射率匹配。LiverClear 非常灵活，可以用于透明化健康和患病的小鼠肝脏，然后进行免疫荧光染色和成像，以可视化完整的 3D 结构，如胆管和肝细胞小管。我们还将此方案进行了改编，以用于透明化人类肝脏。有关此方案的使用和执行的完整详细信息，请参阅 Molina 等人（2021 年）。
