College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.
Cixi Institute of Biomedical Engineering, International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Science (CAS) Key Laboratory of Magnetic Materials and Devices and Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, CAS, Ningbo 315201, P. R. China.
J Mater Chem B. 2022 Nov 16;10(44):9216-9225. doi: 10.1039/d2tb02132c.
The detection of hydrogen peroxide (HO) is of great significance in environmental monitoring, enzymatic reactions, and disease diagnosis. Here we present the peptide-mediated biomimetic synthesis of ultrafine platinum nanowires (PtNWs) on graphene oxide (GO) nanosheets for the formation of functional hybrids, which show high potential for the fabrication of colorimetric and electrochemical sensors for the detection of HO with high performance. A multifunctional peptide with the sequence KIIIIKYWYAF was designed to create peptide nanofibers (PNFs) a controllable self-assembly process, which serves as a bridge between GO nanosheets and PtNWs to form PtNWs-PNFs/GO hybrids. On this basis, a dual-mode sensor platform for both colorimetric and electrochemical sensing of HO was fabricated successfully. The obtained results indicate that the synthesized PtNWs-PNFs/GO hybrids could catalyze the decomposition of HO to generate ˙OH radicals with a significant current response, and the ˙OH radicals are capable of overoxidizing 3,3',5,5',-tetramethylbenzidine (TMB), producing a blue-colored species with a distinct color change for colorimetric sensing. In addition, due to its high catalytic activity, the fabricated PtNWs-PNFs/GO hybrid-based electrochemical sensor exhibits a wider linear detection range of 0.05 μM-15 mM and a low detection limit of 0.0206 μM, which can be applied to detect HO with high selectivity and sensitivity. Our study provides a green and environmentally friendly synthetic strategy for the preparation of biomimetic materials from PtNWs, and the fabricated colorimetric/electrochemical dual-mode HO sensor platform will have a great impact in bioanalysis, environmental monitoring, and biomedicine.
过氧化氢（HO）的检测在环境监测、酶反应和疾病诊断中具有重要意义。在这里，我们提出了一种在氧化石墨烯（GO）纳米片上通过肽介导仿生合成超细微铂纳米线（PtNWs）的方法，用于形成功能杂化材料，这些杂化材料具有用于制备比色和电化学传感器的潜力，可实现对 HO 的高灵敏度检测。设计了一种具有序列 KIIIIKYWYAF 的多功能肽，以创建肽纳米纤维（PNFs），这是一种可控的自组装过程，可作为 GO 纳米片和 PtNWs 之间的桥梁，形成 PtNWs-PNFs/GO 杂化材料。在此基础上，成功构建了用于 HO 的比色和电化学双模式传感的平台。所得结果表明，合成的 PtNWs-PNFs/GO 杂化材料可以催化 HO 的分解，生成具有显著电流响应的˙OH 自由基，并且˙OH 自由基可以过度氧化 3,3',5,5',-四甲基联苯胺（TMB），产生具有明显颜色变化的蓝色物质，用于比色传感。此外，由于其高催化活性，所制备的基于 PtNWs-PNFs/GO 杂化材料的电化学传感器具有更宽的线性检测范围（0.05 μM-15 mM）和更低的检测限（0.0206 μM），可用于高选择性和灵敏度地检测 HO。我们的研究为从 PtNWs 制备仿生材料提供了一种绿色环保的合成策略，并且所构建的比色/电化学双模式 HO 传感器平台将在生物分析、环境监测和生物医学领域产生重大影响。
