神经递质对富含支持细胞培养物中睾酮芳香化作用的刺激效应。

Stimulation effect of neurotransmitters on the aromatization of testosterone by Sertoli cell-enriched cultures.

作者信息

Verhoeven G, Dierickx P, de Moor P

出版信息

Mol Cell Endocrinol. 1979 Mar;13(3):241-53. doi: 10.1016/0303-7207(79)90084-4.

Abstract

The aromatization of testosterone has been studied in Sertoli cell-enriched cultures derived from 19-day-old rats. It was found that, besides dbcAMP and FSH, several neurotransmitters have a stimulatory effect. At a concentration of 10(-5) M, the strongest effects were noted with L-isoproterenol, L-norepinephrine, L-epinephrine and D,L-synephrine. Some stimulation was observed with dopamine, whereas histamine, melatonin and serotonin as well as acetylcholine and pilocarpine were ineffective. Experiments with alpha- and beta-adrenergic antagonists suggest that beta 2- and maybe also alpha-receptors are involved. The same maximal level of stimulation was generally observed for dbcAMP, FSH and isoproterenol. No further increase in activity was obtained with combinations of these compounds. It is concluded that adrenergic agonists may play a role in testicular development and function.

摘要

已在源自19日龄大鼠的富含支持细胞的培养物中研究了睾酮的芳香化作用。结果发现，除了二丁酰环磷腺苷（dbcAMP）和促卵泡激素（FSH）外，几种神经递质也具有刺激作用。在浓度为10⁻⁵ M时，L-异丙肾上腺素、L-去甲肾上腺素、L-肾上腺素和消旋去氧肾上腺素的作用最强。多巴胺有一定的刺激作用，而组胺、褪黑素、5-羟色胺以及乙酰胆碱和毛果芸香碱则无效。使用α和β肾上腺素能拮抗剂的实验表明，可能涉及β₂受体以及α受体。通常观察到dbcAMP、FSH和异丙肾上腺素的刺激水平达到最大值。这些化合物联合使用并未使活性进一步增加。结论是，肾上腺素能激动剂可能在睾丸发育和功能中起作用。