Biswas Deeptarup, Kumari Neha, Lachén-Montes Mercedes, Dutta Sampurna, Agrawal Ishita, Samanta Debabrata, Shenoy Sanjyot Vinayak, Halder Ankit, Fernández-Irigoyen Joaquín, Padhye Advait Rahul, Santamaría Enrique, Srivastava Sanjeeva
Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai400076, India.
Clinical Neuroproteomics Unit, Proteomics Platform, Proteored-ISCIII, Navarrabiomed, Hospital Universitario de Navarra (HUN), Universidad Pública de Navarra (UPNA), Navarra Institute for Health Research (IdiSNA), 31008Pamplona, Spain.
J Proteome Res. 2023 Apr 7;22(4):1043-1055. doi: 10.1021/acs.jproteome.2c00244. Epub 2022 Nov 1.
Post-translational modifications (PTMs) are one of the compulsive and predominant biological processes that regulate the diverse molecular mechanism, modulate the onset of disease, and are the reason behind the functional diversity of proteins. Despite the widespread research findings in neuroproteomics, one of the key drawbacks has been the lack of proteome-level knowledge of hemispheric lateralization. We have investigated the proteome level expression in different neuroanatomical regions under the Human Brain Proteome Project (HBPP) and developed the global interhemispheric brain proteome map (Brainprot) earlier. Furthermore, this study has extended to decipher the phosphoproteome map of human brain interhemispheric regions through high-resolution mass spectrometry. The phosphoproteomics examination of 12 unique interhemispheric neurological brain regions using Orbitrap fusion liquid chromatography with tandem mass spectrometry provided comprehensive coverage of 996 phosphoproteins, 2010 phosphopeptides, and 3567 phosphosites. Moreover, interhemispheric phosphoproteome profiling has been categorized according to synaptic ontologies and interhemispheric expression to understand the functionality. Finally, we have integrated the phosphosites data under the PhosphoMap section in the Inter-Hemispheric Brain Proteome Map Portal (https://www.brainprot.org/) for the advancement and support of the ongoing neuroproteomics research worldwide. Data is available via ProteomeXchange with the identifier PXD031188.
翻译后修饰（PTMs）是调控多种分子机制、调节疾病发生且导致蛋白质功能多样性的重要且主要的生物学过程之一。尽管在神经蛋白质组学方面有广泛的研究发现，但关键缺点之一是缺乏关于半球偏侧化的蛋白质组水平的知识。我们在人类大脑蛋白质组计划（HBPP）下研究了不同神经解剖区域的蛋白质组水平表达，并较早地绘制了全球大脑半球间蛋白质组图谱（Brainprot）。此外，本研究通过高分辨率质谱进一步扩展，以解析人类大脑半球间区域的磷酸化蛋白质组图谱。使用轨道阱融合液相色谱串联质谱对12个独特的大脑半球间神经区域进行磷酸化蛋白质组学检测，全面覆盖了996种磷酸化蛋白质、2010种磷酸化肽段和3567个磷酸化位点。此外，已根据突触本体和半球间表达对半球间磷酸化蛋白质组进行分类，以了解其功能。最后，我们将磷酸化位点数据整合到半球间大脑蛋白质组图谱门户（https://www.brainprot.org/）的PhosphoMap部分，以推动和支持全球正在进行的神经蛋白质组学研究。数据可通过ProteomeXchange获得，标识符为PXD031188。
