State Key Laboratory of North China Crop Improvement and Regulation, Hebei Sub-Center for National Maize Improvement Center, College of Agronomy, Hebei Agricultural University, Hebei, Baoding 071001, China.
College of Agronomy, Qingdao Agricultural University, Shandong, Qingdao 266109, China.
BMC Genom Data. 2022 Nov 1;23(1):76. doi: 10.1186/s12863-022-01091-5.
Stalk lodging is one of the main factors affecting maize (Zea mays L.) yield and limiting mechanized harvesting. Developing maize varieties with high stalk lodging resistance requires exploring the genetic basis of lodging resistance-associated agronomic traits. Stalk strength is an important indicator to evaluate maize lodging and can be evaluated by measuring stalk rind penetrometer resistance (RPR) and stalk buckling strength (SBS). Along with morphological traits of the stalk for the third internodes length (TIL), fourth internode length (FIL), third internode diameter (TID), and the fourth internode diameter (FID) traits are associated with stalk lodging resistance.
In this study, a natural population containing 248 diverse maize inbred lines genotyped with 83,057 single nucleotide polymorphism (SNP) markers was used for genome-wide association study (GWAS) for six stalk lodging resistance-related traits. The heritability of all traits ranged from 0.59 to 0.72 in the association mapping panel. A total of 85 significant SNPs were identified for the association mapping panel using best linear unbiased prediction (BLUP) values of all traits. Additionally, five candidate genes were associated with stalk strength traits, which were either directly or indirectly associated with cell wall components.
These findings contribute to our understanding of the genetic basis of maize stalk lodging and provide valuable theoretical guidance for lodging resistance in maize breeding in the future.
茎倒伏是影响玉米（Zea mays L.）产量和限制机械化收获的主要因素之一。开发具有高茎倒伏抗性的玉米品种需要探索与倒伏抗性相关的农艺性状的遗传基础。茎秆强度是评估玉米倒伏的一个重要指标，可以通过测量茎皮穿刺阻力（RPR）和茎弯曲强度（SBS）来评估。与第三节间长度（TIL）、第四节间长度（FIL）、第三节间直径（TID）和第四节间直径（FID）等形态学特征一起，与茎倒伏抗性有关。
本研究利用含有 248 个不同玉米自交系的自然群体，对 83057 个单核苷酸多态性（SNP）标记进行了基因型分析，对 6 个与茎倒伏抗性相关的性状进行了全基因组关联研究（GWAS）。在关联作图群体中，所有性状的遗传力在 0.59 到 0.72 之间。利用所有性状的最佳线性无偏预测（BLUP）值，对关联作图群体共鉴定出 85 个显著 SNP。此外，还鉴定到 5 个与茎强度性状相关的候选基因，这些基因要么直接，要么间接地与细胞壁成分有关。
这些发现有助于我们了解玉米茎倒伏的遗传基础，并为未来玉米抗倒伏育种提供有价值的理论指导。
