Fine J D, Redmar D A, Goodman A L
Arch Dermatol. 1987 Sep;123(9):1174-8.
Recent studies have shown an orderly sequence of expression of structural antigens within the skin basement-membrane zone of the human fetus. Interestingly, these findings differ from those reported following split-thickness would induction in Yorkshire pigs. To readdress this apparent disparity, as well as to further extend such studies to include other basement-membrane antigens more recently identified, we produced split-thickness wounds in a primate species and serially examined the wounds by immunofluorescence technique for the expression of seven antigens normally found in intact primate skin basement membrane. By the fourth day following wounding, laminin, type-IV collagen, and fibronectin were all detectable along the dermoepidermal junction. In contrast, bullous pemphigoid antigen, epidermolysis bullosa acquisita antigen, cicatricial pemphigoid antigen, and KF-1 antigen were undetectable until days 6, 8, 10, and 11, respectively. These latter findings are in complete agreement with those previously reported in the developing human fetus. On the basis of the results of this study, we would suggest that primate rather than pig skin may be a more appropriate animal model for wound-healing studies. These data are consistent with previous reports suggesting that the basement-membrane antigens recognized by bullous pemphigoid and cicatricial pemphigold autoantibodies are indeed distinct. Finally, these data bring into question the functional role of bullous pemphigoid antigen during the early phase of wound healing in humans.
近期研究表明，人类胎儿皮肤基底膜区内结构抗原的表达具有有序序列。有趣的是，这些发现与在约克郡猪身上进行的中厚皮片创伤诱导后所报告的结果不同。为了重新审视这一明显差异，并进一步扩展此类研究以纳入最近鉴定出的其他基底膜抗原，我们在一种灵长类动物身上制造了中厚皮片伤口，并通过免疫荧光技术对伤口进行连续检查，以检测完整灵长类动物皮肤基底膜中通常存在的七种抗原的表达情况。受伤后第四天，在真皮表皮交界处可检测到层粘连蛋白、IV型胶原蛋白和纤连蛋白。相比之下，大疱性类天疱疮抗原、获得性大疱性表皮松解症抗原、瘢痕性类天疱疮抗原和KF-1抗原分别直到第6天、第8天、第10天和第11天才被检测到。后一组发现与之前在发育中的人类胎儿中报告的结果完全一致。基于本研究结果，我们认为灵长类动物而非猪的皮肤可能是伤口愈合研究更合适的动物模型。这些数据与之前的报告一致，表明大疱性类天疱疮和瘢痕性类天疱疮自身抗体识别的基底膜抗原确实不同。最后，这些数据使人质疑大疱性类天疱疮抗原在人类伤口愈合早期阶段的功能作用。
