Zaker Sayed Rasoul, Nili Hassan, Asasi Keramat
Department of Biology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, Iran.
Avian Diseases Research Center, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Vet Res Forum. 2022 Sep;13(3):439-441. doi: 10.30466/vrf.2020.119136.2818. Epub 2022 Sep 15.
Avian influenza virus subtype HN is widely circulating around the globe affecting many species of animal including mammals and birds as well as human beings. The virus has pandemic potential due to segmented nature of the viral genome. Ultra-structural features of apoptosis in field and experimental infection of HN avian influenza virus were studied. Freshly dead birds from affected broiler farms and experimentally infected broiler chickens with HN subtypes were subjected to routine necropsy. Post-mortem findings in different organs were recorded. Appropriate specimens from the trachea were taken for electron microscopy studies. In electron microscopy study, frequent apoptotic bodies were observed in the epithelial cells of the trachea. Increase of antibody titer to HN virus following challenge with the virus in experimental group indicates that the infectious cycle has been initiated in the affected birds.
禽流感病毒HN亚型在全球广泛传播，影响包括哺乳动物、鸟类以及人类在内的许多动物物种。由于病毒基因组的分段特性，该病毒具有大流行的潜力。对HN禽流感病毒田间感染和实验感染中的细胞凋亡超微结构特征进行了研究。对来自受影响肉鸡场的刚死亡的鸡以及用HN亚型进行实验感染的肉鸡进行常规尸检。记录不同器官的死后检查结果。从气管采集合适的标本用于电子显微镜研究。在电子显微镜研究中，在气管上皮细胞中观察到频繁出现的凋亡小体。实验组在用该病毒攻击后，针对HN病毒的抗体滴度升高，这表明受感染禽类已开始感染循环。
