Tian Xin, He Xudong, Xu Jinpeng, Yang Zhengfei, Fang Weiming, Yin Yongqi
College of Food Science and Engineering, Yangzhou University Yangzhou Jiangsu 225009 People's Republic of China +86-514-89786551 +86-514-89786551
Yangzhou Center for Food and Drug Control Yangzhou Jiangsu 225009 People's Republic of China.
RSC Adv. 2022 Oct 13;12(45):29214-29222. doi: 10.1039/d2ra05289j. eCollection 2022 Oct 11.
Phenolic acid is a physiologically active substance that has a variety of effects on humans. Barley sprouts are often used as food ingredients to enrich phenolic acids and to further produce functional foods rich in phenolic acids. In this study, the mechanism of Ca involvement in regulating phenolic acid biosynthesis and plant growth in barley by melatonin (MT) under NaCl stress was investigated. According to the studies, MT (25 μM) increased total calcium content, induced Ca burst, and up-regulated the gene expression of calcium-regulated protein-dependent protein kinase and calcium-binding protein transcription-activating protease in NaCl-stressed (60 mM) barley. Exogenous MT and its combined CaCl (0.4 mM) significantly promoted phenolic acid biosynthesis by increasing the activity of C4H and PAL, and induced gene expression of and . The addition of exogenous CaCl and MT caused systemic tolerance in NaCl-stressed barley, as determined by a decrease in the fluorescence intensity of hydrogen peroxide and oxygen radical anions as well as an enhancement in the antioxidant enzyme, thus significantly increasing sprout length and fresh weight. In addition, combined use of MT with Ca antagonists (lanthanum chloride or ethylene glycol tetraacetic acid), impaired all impacts as mentioned above. These findings imply that Ca participated in MT-induced phenolic acid biosynthesis and growth improvement in NaCl-stressed barley.
酚酸是一种对人体具有多种作用的生理活性物质。发芽大麦常被用作食品原料，以富集酚酸并进一步生产富含酚酸的功能性食品。本研究探讨了褪黑素（MT）在NaCl胁迫下调控大麦酚酸生物合成和植物生长过程中钙的作用机制。研究表明，MT（25μM）增加了总钙含量，诱导了钙爆发，并上调了NaCl胁迫（60 mM）大麦中钙调节蛋白依赖性蛋白激酶和钙结合蛋白转录激活蛋白酶的基因表达。外源MT及其与CaCl₂（0.4 mM）的组合通过提高C4H和PAL的活性显著促进了酚酸的生物合成，并诱导了相关基因的表达。添加外源CaCl₂和MT使NaCl胁迫下的大麦产生系统耐受性，这通过过氧化氢和氧自由基阴离子荧光强度的降低以及抗氧化酶的增强得以确定，从而显著增加了芽长和鲜重。此外，MT与钙拮抗剂（氯化镧或乙二醇四乙酸）联合使用会削弱上述所有影响。这些发现表明，钙参与了MT诱导的NaCl胁迫下大麦酚酸生物合成和生长改善过程。
