Tian Xin, Zhang Renjiao, Yang Zhengfei, Zhu Jiangyu, Fang Weiming, Yang Runqiang, Yin Yongqi
College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Front Nutr. 2024 Jun 4;11:1403293. doi: 10.3389/fnut.2024.1403293. eCollection 2024.
Phenolic acids are secondary metabolites in higher plants, with antioxidant, anticancer, and anti-aging effects on the human body. Therefore, foods rich in phenolic acids are popular. Methyl jasmonate (MeJA) promoted phenolic acids accumulation but also inhibited sprout growth. Melatonin (MT) was a new type of plant hormone that not only alleviated plants' abiotic stress, but also promoted the synthesis of plant-stimulating metabolism. This study aimed to elucidate the mechanism of exogenous MT on the growth and development, and phenolic acids metabolism of barley sprouts under MeJA treatment. The results showed that MT increased the phenolic acids content in sprouts by increasing the activities of phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase, and up-regulating the gene expression of , , , and . MT attenuated the growth inhibition of barley sprouts under MeJA stress by increasing the activities of regulated antioxidant enzymes and the expression of their corresponding genes. Furthermore, MT increased the NO content and induced Ca burst in barley sprouts under MeJA stress. These events were inhibited by DL-4-Chlorophenylalanine. These results suggested that MT ameliorated growth inhibition and promoted the biosynthesis of phenolic acids in barley sprouts under MeJA stress.
酚酸是高等植物中的次生代谢产物，对人体具有抗氧化、抗癌和抗衰老作用。因此，富含酚酸的食物很受欢迎。茉莉酸甲酯（MeJA）促进酚酸积累，但也抑制芽苗生长。褪黑素（MT）是一种新型植物激素，不仅能缓解植物的非生物胁迫，还能促进植物刺激代谢的合成。本研究旨在阐明外源MT对MeJA处理下大麦芽苗生长发育及酚酸代谢的影响机制。结果表明，MT通过提高苯丙氨酸解氨酶和肉桂酸4-羟化酶的活性，上调、、、和的基因表达，增加了芽苗中酚酸的含量。MT通过提高抗氧化酶的活性及其相应基因的表达，减轻了MeJA胁迫下大麦芽苗的生长抑制。此外，MT增加了MeJA胁迫下大麦芽苗中的NO含量并诱导了Ca2+爆发。这些事件被DL-4-氯苯丙氨酸抑制。这些结果表明，MT缓解了MeJA胁迫下大麦芽苗的生长抑制并促进了酚酸的生物合成。
