N-乙酰葡萄糖胺诱导马克斯克鲁维酵母增强对扩展青霉的生防效果。
Enhancement of biocontrol efficacy of Kluyveromyces marxianus induced by N-acetylglucosamine against Penicillium expansum.
机构信息
National Engineering Laboratory of Intelligent Food Technology and Equipment, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
National Engineering Laboratory of Intelligent Food Technology and Equipment, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
出版信息
Food Chem. 2023 Mar 15;404(Pt B):134658. doi: 10.1016/j.foodchem.2022.134658. Epub 2022 Oct 19.
This research aimed to investigate the effect of N-acetylglucosamine (GlcNAc) on the biocontrol activity of Kluyveromyces marxianus and involved possible mechanisms. The results indicated that 0.5% GlcNAc significantly improved the antagonistic efficacy of K. marxianus against Penicillium expansum. GlcNAc supplementation led to increases of biocontrol-related enzymes and stress-resistant substances in K. marxianus including chitinase, β-1,3-glucanase, trehalose and proline, being beneficial for yeasts vigor maintenance under adverse circumstances. The antioxidative-related enzyme activities in K. marxianus, such as superoxide dismutase (SOD), catalase (CAT) and others, were increased by GlcNAc along with more quickly excess reactive oxygen species (ROS) scavenging. GlcNAc resulted in an enhancement of proliferative capacity in K. marxianus according to the transcriptomic analysis. Consequently, GlcNAc contributed to improvements of K. marxianus in the environmental adaptability and stress resistance. Our study demonstrated that both GlcNAc and K. marxianus could be regarded as potential candidates for postharvest technology application.
本研究旨在探讨 N-乙酰氨基葡萄糖（GlcNAc）对马克斯克鲁维酵母生物防治活性的影响，并探讨可能的机制。结果表明，0.5%GlcNAc 显著提高了马克斯克鲁维酵母对扩展青霉的拮抗作用。GlcNAc 补充使马克斯克鲁维酵母中的生物防治相关酶和应激抗性物质增加，包括几丁质酶、β-1,3-葡聚糖酶、海藻糖和脯氨酸，有利于酵母在不利环境下维持活力。超氧化物歧化酶（SOD）、过氧化氢酶（CAT）等抗氧化相关酶的活性也因 GlcNAc 的存在而增加，同时能更快地清除过量的活性氧（ROS）。GlcNAc 还通过转录组分析促进了马克斯克鲁维酵母增殖能力的提高。因此，GlcNAc 有助于提高马克斯克鲁维酵母的环境适应性和抗应激能力。本研究表明，GlcNAc 和马克斯克鲁维酵母均可作为采后技术应用的潜在候选物。
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