棕榈油和棕榈硬脂对低脂和高脂饮食 C57BL/6J 小鼠盲肠和粪便微生物群的影响。

Effects of palm olein and palm stearin on cecal and fecal microbiota of C57BL/6J mice under low and high fat intakes.

机构信息

School of Food Science and Technology, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510640, China; Department of Microbial Pathogenesis, School of Dentistry, University of Maryland - Baltimore, Baltimore, MD 21201, USA; School of Food Science and Engineering, Hainan University, Haikou 570228, China.

School of Chemical Engineering and Energy Technology, Dongguan University of Technology, Dongguan 523808, China.

出版信息

Food Chem. 2023 Mar 15;404(Pt B):134693. doi: 10.1016/j.foodchem.2022.134693. Epub 2022 Oct 19.

DOI:10.1016/j.foodchem.2022.134693

PMID:36323033

Abstract

This work aimed to study the effects of dietary lipid composition and content on cecal and fecal microbiota of mice fed the following diets for 8 weeks: palm olein (PO)-based low-fat diet, PO-based high-fat diet, palm stearin (PS)-based low-fat diet, and PS-based high-fat diet. Increasing the dietary PS level favored the growth of Firmicutes over Bacteroidetes in the cecum and feces. In addition, it significantly elevated the total lipid (p < 0.01) and bile acid content (p < 0.01) in feces, resulting in the enrichment of fat-degrading and bile-acid tolerant genera within the families Ruminococcaceae and Lachnospiraceae. Although increasing the PO intake also caused obesity in mice, it did not affect the microbial structure. When fat intake is constant, only at a high-fat level can PS (vs PO) induce the above-mentioned microbial shifts. These results highlighted the combined roles of lipid quality and quantity on the gut microbiota.

摘要

本研究旨在探讨不同膳食脂质组成和含量对喂食以下 8 周饮食的小鼠盲肠和粪便微生物群的影响：棕榈油（PO）为基础的低脂肪饮食、PO 为基础的高脂肪饮食、棕榈硬脂精（PS）为基础的低脂肪饮食和 PS 为基础的高脂肪饮食。增加膳食 PS 水平有利于盲肠和粪便中厚壁菌门相对于拟杆菌门的生长。此外，它还显著增加了粪便中的总脂质（p<0.01）和胆汁酸含量（p<0.01），导致瘤胃球菌科和lachnospiraceae 家族内的脂肪降解和胆汁酸耐受属的富集。尽管增加 PO 摄入也会导致小鼠肥胖，但它不会影响微生物结构。当脂肪摄入量保持不变时，只有在高脂肪水平下，PS（与 PO 相比）才能引起上述微生物变化。这些结果强调了脂质质量和数量对肠道微生物群的综合作用。