Loo Yit Tao, Howell Kate, Suleria Hafiz, Zhang Pangzhen, Liu Siyao, Ng Ken
School of Agriculture & Food, Faculty of Veterinary & Agricultural Sciences, The University of Melbourne, Victoria 3010, Australia.
School of Agriculture & Food, Faculty of Veterinary & Agricultural Sciences, The University of Melbourne, Victoria 3010, Australia.
Food Chem. 2023 Mar 15;404(Pt B):134721. doi: 10.1016/j.foodchem.2022.134721. Epub 2022 Oct 26.
This study investigated the effects of the sugarcane flavones diosmin, diosmetin, luteolin, and tricin, and their interactions with sugarcane fiber on the modulation of gut microbiota using in vitro batch fermentation. The alteration of fecal fermentation bacterial profile was analyzed using 16S rRNA sequencing data, while the bioavailability of fiber was indicated by short-chain fatty acid (SCFA) production and metabolism of polyphenols was measured directly by phenolic metabolites. Application of diosmin, diosmetin, luteolin, and tricin without fiber had no significant effect on the overall microbiota profile after 24 h of fermentation. When fiber alone was added, total SCFA production increased, specifically that of propionic and valeric acids. However, when flavones were combined with fiber, synergistic effects on the modulation of relative abundances of different bacterial taxa was noted. In particular, the proportion of Prevotella spp. was significantly increased by the combinations of diosmin, luteolin, and tricin with fiber.
本研究利用体外批次发酵，研究了甘蔗黄酮类化合物地奥司明、香叶木素、木犀草素和小麦黄素及其与甘蔗纤维的相互作用对肠道微生物群的调节作用。利用16S rRNA测序数据分析粪便发酵细菌谱的变化，而纤维的生物利用度通过短链脂肪酸（SCFA）的产生来表示，多酚的代谢则通过酚类代谢物直接测定。在发酵24小时后，单独添加地奥司明、香叶木素、木犀草素和小麦黄素对整体微生物群谱没有显著影响。单独添加纤维时，总SCFA产量增加，尤其是丙酸和戊酸的产量。然而，当黄酮类化合物与纤维结合时，发现对不同细菌类群相对丰度的调节具有协同作用。特别是，地奥司明、木犀草素和小麦黄素与纤维的组合显著增加了普雷沃氏菌属的比例。
