一种新型高选择性 Fe(III)荧光共振能量转移传感器及其 DFT 机理评价。
A novel highly selective FRET sensor for Fe(III) and DFT mechanistic evaluation.
机构信息
Department of Basic Science and Physical Education, Faculty of Science at Sriracha, Kasetsart University Sriracha Campus, Chonburi 20230, Thailand.
Department of Chemistry and Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Feb 5;286:122031. doi: 10.1016/j.saa.2022.122031. Epub 2022 Oct 22.
A novel FRET-based sensor has been designed and developed through the conjugation of naphthyl and rhodamine via propylamine spacer, Naph-Rh. The naphthyl moiety serves as a FRET donor due to its emission spectrum overlapping with the rhodamine B absorption band. Naph-Rh exhibited a selectivity for sensing Fe over other metal ions with a visual color change and fluorescent enhancement. The ratio of the Naph-Rh and Fe was determined to be 1:1 based on Job's plot analysis with a detection limit of 83 nM. The probe exhibited a linear response to Fe in the range of 0-120 μM. Furthermore, the density functional theory (DFT) calculations of Naph-Rh were carried out to rationalize the design and portray the plausible Fe sensing mechanism.
一种新型的荧光共振能量转移（FRET）传感器通过丙胺间隔臂将萘基和罗丹明连接起来，设计并开发成功，该传感器被命名为 Naph-Rh。由于萘基部分的发射光谱与罗丹明 B 的吸收带重叠，因此它可以作为 FRET 供体。Naph-Rh 对 Fe 具有选择性，能够通过肉眼观察到颜色变化和荧光增强来检测 Fe，而对其他金属离子没有响应。通过 Job 分析图可知，Naph-Rh 与 Fe 的比例为 1:1，检测限为 83 nM。该探针对 Fe 的线性响应范围为 0-120 μM。此外，还进行了 Naph-Rh 的密度泛函理论（DFT）计算，以合理设计并描绘可能的 Fe 传感机制。
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